聚(NIPAAM-EDMA)整体柱的制备及其对尿样中氢化可的松的富集分析  

Preparation of poly(NIPAAM-EDMA) monolithic column for enriching and analyzing hydrocortisone from urine

作　　者：贾艳花[1] 刘海燕[1] 杨宁[1] 段艳慧[1] 杨更亮[1] 

机构地区：[1]河北大学药学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2012年第2期154-158,共5页Journal of Hebei University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(21175031);河北省科技厅资助项目(11966411D);河北省教育厅资助项目(2008416;ZD2010234);科技部"重大新药创制"科技重大专项"十二五"计划第二批课题(2012ZX09103-101-057);河北大学自然科学基金资助项目(2009-165).42

摘　　要：以N-异丙基丙烯酰胺(NIPAAM)为单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,制备了一种能有效去除尿样中的大分子物质,并能同时富集尿样中氢化可的松的整体柱.实验结果表明:氢化可的松在5～30mg/L线性关系良好(R=0.999 3,n=6),检测限为0.012mg/L;绝对回收率79%～83%;日内、日间精密度均<5.0%,整体柱能够很好地富集尿样中的氢化可的松,并且能很好地除去尿样中的大分子物质及杂质,能满足分析的要求.A new monolithic column have been prepared which was composed of copolymer that the N- isopropylacrylamide （N-IPAAM） was the monomer and the ethyleneglycoldimethacrylate（EDMA） was the cross-linker. The monolith was used for removing the macromoleculer and enriching the hydrocortisone in urine sample. The result showed that hydrocortisone had a good linear（R=0. 999 3, n=6） when the con- centration of the hydrocortisone in the range of 5-30 mg/L , the limit of detection（LOD） was 0. 012 mg/L. The absolutely recovery was 79%-83%. The inter-day RSD and intra-day RSD was less than 5%. This method is conforming to analysis.

关 键 词：N-异丙基丙烯酰胺 整体柱 富集分析 氢化可的松 

分 类 号：R917[医药卫生—药物分析学]