广西猪源H9N2亚型流感病毒全基因组序列分析  

Complete genome sequence analysis of H9N2 subtype influenza virus from swine in Guangxi

作　　者：伍和明 韦达有 易春华 徐贤坤[2] 何奇松[2] 孙翔翔[2] 蒋家霞[2] 付薇[2] 熊毅[2] 

机构地区：[1]桂林市动物疫病预防控制中心,广西桂林541002 [2]广西动物疫病预防控制中心,南宁530001

出　　处：《南方农业学报》2012年第3期389-392,共4页Journal of Southern Agriculture

基　　金：广西科技攻关项目(2010GXNSFD013033)

摘　　要：【目的】了解广西猪源H9N2亚型流感病毒的来源及变异情况,为有效防控广西猪源H9N2亚型流感病毒奠定基础。【方法】运用RT-PCR对广西分离株A/SW/GX/S11/05(H9N2)进行全基因组扩增,并克隆测序及对比分析。【结果】扩增获得的HA、NA、NP、M、NS、PB1、PB2和PA基因片段长度分别为1683、1401、1497、1027、890、2233、2280和2151bp,其推导氨基酸分别为561、467、499、349、338、744、760和717aa。经过序列分析,发现各基因片段的核苷酸及其推导氨基酸与A/Bird/GX/62/05株的同源性最高,分别为96.2%～100.0%和98.5%～99.6%;系统进化分析也表明A/SW/GX/S11/05株与A/Bird/GX/62/05株的亲缘关系最近。【结论】广西猪源H9N2亚型流感病毒属于欧亚谱系的北京亚系,且在一定时间内不同宿主间均有感染,在今后的流感病毒防治工作中,跨种属传播是一个不可忽视的问题。[Objective]The study was undertaken to investigate the sources and variation of H9N2 influenza virus from swine in Guangxi and to provide a reference for its prevention and control.[Method]The genomic DNA of influenza virus strain A/SW/GX/S11/05（H9N2）,was isolated,amplified,cloned and sequenced.[Result]The length of HA,NA,NP,M,NS,PB1,PB2 and PA genes was 1683,1401,1497,1027,890,2233,2280 and 2151 bp,respectively,and the length of deduced amino acids was 561,467,499,349,338,744,760 and 717 aa.The results revealed that nucleotide and deduced amino acids of the strain had 96.2-100.0% and 98.5-99.6% homology with strain A/Bird/GX/62/05,respectively.The systemic evolutionary analysis also proved that the genetic relationship between strain SW/GX/S11/05 and A/Bird/GX/62/05 was the closest.[Conclusion]The H9N2 subtype influenza virus from swine（collected in Guangxi） belonged to Beijing subspecies of Eurasian pedigree,and could infect to various hosts in a certain period.Consequently,trans-species and genus transmission should be taken into account in terms of prevention and control of the influenza viruses in future.

关 键 词：猪源流感病毒 H9N2亚型 全基因组 序列分析 广西 

分 类 号：S852.659.5[农业科学—基础兽医学] S858.28[农业科学—兽医学]