拟南芥抗旱突变体的筛选和鉴定  

Screening and identification of drought-resistant mutants in Arabidopsis

作　　者：张娇娇[1] 江力[1] 杨杰[2] 慈凌坤[1] 卢云峰[1] 曹树青[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]合肥师范学院生命科学系,安徽合肥230061

出　　处：《合肥工业大学学报（自然科学版）》2012年第4期531-535,共5页Journal of Hefei University of Technology：Natural Science

基　　金：安徽省高等学校省级自然科学研究重大资助项目(KJ2011ZD08)

摘　　要：文章采用不同浓度的甘露醇模拟干旱胁迫,以发芽率为筛选指标在拟南芥突变体库中筛选抗旱突变体,筛选出2株候选突变体,最终得到1株稳定突变体vem1,通过表型和生理生化鉴定,确定为抗旱突变体。该研究为抗旱基因克隆及功能分析奠定基础,对于揭示植物抗旱的分子机理具有重要的理论意义。The wild-type and mutant lines of Arabidopsis were treated with the mannitol at different concentra- tions to simulate drought stress to screen drought-resistant mutants by germination capacity analysis. There were two mutant lines as candidates and eventually one stable mutant line veml was obtained. The phenotype and biochemical identification showed that the veml was drought-resistant. The study provides a basis for the cloning and functional analysis of drought-resistant genes, and it is of important theoretical significance to reveal the molecular mechanisms of the tolerance of plants to drought.

关 键 词：抗旱 突变体 筛选 鉴定 拟南芥 

分 类 号：Q754[生物学—分子生物学]