正义和反义Calpastatin cDNA真核表达载体的构建  

THE CONSTRUCTION OF pRC/RSV SENSE AND ANTISENSE CALPASTATIN cDNA EXPRESSION VECTORS

作　　者：杜敏[1] 南庆贤[1] 朱美君[2] 张曼夫[2] 

机构地区：[1]中国农业大学食品学院,北京100094 [2]中国农业大学生物学院,北京100094

出　　处：《畜牧兽医学报》2000年第2期97-101,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金!(编号 396 70 5 48);International Foundation for Science(瑞典)资助

摘　　要：本研究利用真核表达载体 pRC/RSV ,将位于 pBS SK质粒中的CalpastatincDNA以正反两个方向构入载体 ;经酶切分析 ,构建的质粒结构正确。所构建质粒 pRC/RSV正义、反义CalpastatincDNA两个表达载体 ,为进一步转化肌细胞 ,人为控制Calpastatin基因的表达 ,从而控制Calpain活性 ,深入研究钙蛋白酶在肌肉发育及肉嫩化中的作用打下基础。WT5BZ] Calpain system has been suggested to play a key role in myoblast fusion and muscle growth.Possibly,we can enhance the growth of muscle through controlling the activity of calpains.Calpastatin is the endogenous inhibitor of calpains which regulate calpain activity in vivo .This research employs eukaryotic expression vector pRC/RSV and Calpastatin cDNA in pBS SK to construct two expression vectors in which calpastatin cDNA are inserted in sense and antisense direction respectively.Endonuclease restriction analysis proved that the two plasmids were correctly constructed.Those plasmids will be useful in regulating the activities of calpains by controlling the expression of calpastatin gene,and facilitate researches in the role of calpain system in muscle growth and meat tenderization. [WT5HZ]

关 键 词：钙蛋白酶 真核表达载体 肌肉发育 基因表达 家畜 

分 类 号：S852.2[农业科学—基础兽医学] S813.2[农业科学—兽医学]