应用套入式聚合酶链反应(Nested-PCR)检测蓝舌病病毒的研究  

NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF BLUETONGUE VIRUS

作　　者：马洪超[1] 孙淑芳[1] 陶茂辉 尹燕博[1] 郭福生[1] 龚振华[1] 蔡丽娟[1] 蒋正军[1] 丁有胜 黄运生 陈书琨 

机构地区：[1]农业部动物检疫所,266032 [2]深圳动植物检疫局,518010

出　　处：《畜牧兽医学报》2000年第2期145-149,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业部"九五"重点攻关课题

摘　　要：本实验建立了一种Nested PCR方法。应用该方法检测 5株标准株蓝舌病病毒 (T4、T10、T11、T16、T2 0 )和 5株国内蓝舌病病毒分离株 (CF4、AF6、Z1、H1、G14) ,检测结果均为阳性 ,而检测相关环状病毒 (EHD2、EHD6、Ibraki) ,检测结果均为阴性。研究证明 ,此方法可检测到3 5fg的BTV—RNA ,灵敏度较高。该方法成功区分了蓝舌病病毒和相关环状病毒 ,比血清学方法更加优越 ,在临床检测方面具有广阔的应用前景。WT5BZ] A Nested PCR method was established,and used for the detection of five standard BTV strains (T4,T10,T11,T16,T20) and five China local BTV isolates (Cf4,Af6,Z1,H1,G14) all with positive result We also tested the related orbiviruses (es EHD2,EHD6,Ibraki),resulting in negative reaction The study proved that the PCR method was highly sensitive,The detective rate can reach 3 5fg BTV RNA It’s a more advanced method compared with the AGID for it can distinguish BTV from other orbiviruses The higher sensitivity and specificity of the PCR method is more useful for clinical diagnosis [WT5HZ]

关 键 词：家畜 蓝舌病病毒 NESTED-PCR 检测方法 

分 类 号：S855.3[农业科学—临床兽医学] S854.43[农业科学—兽医学]