(Z)-5-(4-methoxybenzylidene)thiazolidine-2,4-dione protects rats from carbon tetrachloride-induced liver injury and fibrogenesis  

作　　者：Zhi-Zhi Chen Zheng-Lin Wang Chong-Yang Deng Hao Zheng Xian-Huo Wang Liang Ma Xia Ye Ying-Hua Ma Cai-Feng Xie Li-Juan Chen Yu-Quan wei 

机构地区：[1]State Key Labora-tory of Biotherapy and Cancer Center,West China Hospital,WestChina Medical School,Sichuan University,Chengdu 610041,Sichuan Province,China

出　　处：《World Journal of Gastroenterology》2012年第7期654-661,共8页世界胃肠病学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China and National Key Technologies R and D Program of the 11th five-year plan,No.2009ZX09501-015

摘　　要：AIM： To evaluate the hepatoprotective roles of （Z）- 5-（4-methoxybenzylidene）thiazolidine-2,4-dione （SKLB010） against carbon tetrachloride （CCI4）-induced acute and chronic liver injury and its underlying mecha- nisms of action.AIM:To evaluate the hepatoprotective roles of (Z)5-(4-methoxybenzylidene)thiazolidine-2,4-dione (SKLB010) against carbon tetrachloride (CCl 4)-induced acute and chronic liver injury and its underlying mechanisms of action.METHODS:In the first experiment,rats were weighed and randomly divided into 5 groups (five rats in each group) to assess the protective effect of SKLB010 on acute liver injury.For induction of acute injury,rats were administered a single intraperitoneal injection of 2 mL/kg of 50% (v/v) CCl 4 dissolved in olive oil (1:1).Group 1 was untreated and served as the control group;group 2 received CCl 4 for induction of liver injury and served as the model group.In groups 3,4 and 5,rats receiving CCl 4 were also treated with SKLB010 at doses of 25,50 and 100 mg/kg,respectively.Blood samples were collected at 6,12 and 24 h after CCl 4 intoxication to determine the serum activity of alanine amino transferase.Tumour necrosis factor-α (TNF-α),interleukin-1β (IL-1β) were determined using enzyme-linked immunosorbent assay.At 24 h after CCl 4 injection,liver fibrogenesis was evaluated by hematoxylin-eosin (HE) staining and immunohistochemical analyses.Cytokine transcript levels of TNF-α,IL-1β and inducible nitric oxide synthase in the liver tissues of rats were measured using a reverse transcriptase reverse transcription-polymerase chain reaction technique.In the second experiment,rats were randomly divided into 2 groups (15 rats in each group),and liver injury in the CCl 4-administered groups was induced by a single intraperitoneal injection of 2 mL/kg of 50% (v/v) CCl 4 dissolved in olive oil (1:1).The SKLB010-treated groups received oral 100 mg/kg SKLB010 before CCl 4 administration.Five rats in each group were sacrificed at 2 h,6 h,12 h after CCl 4 intoxication and small fortions of livers were rapidly frozen for extraction of total RNA,hepatic proteins and glutathione (GSH) assays.In the hepatic fibrosis model group,rats were randomly divided into 2 groups (5 rats each group).Rats were injected int

关 键 词：Anti-inflammatory effects Anti-oxidativeeffects （Z）-5-（4-methoxybenzylidene） thiazolidine-2 4-dione （SKLB010） against carbon tetrachloride Fibro-genesis Hepatitis Nuclear factor-KB SKLB010 

分 类 号：O625.31[理学—有机化学] TQ222.214[理学—化学]