脱细胞血管基质材料同种异体移植10个月研究  

Acellular vascular matrix materials for allogeneic transplantation:A 10-month study

作　　者：范恒华[1] 伍骥[1] 张伯勋[2] 陈明智[1] 张新合[1] 朱克顺[1] 

机构地区：[1]解放军空军总医院骨科,北京市100142 [2]解放军总医院骨科,北京市100187

出　　处：《中国组织工程研究》2012年第12期2149-2152,共4页Chinese Journal of Tissue Engineering Research

基　　金：北京市首都医学科学发展基金资助(2005-3094)~~

摘　　要：背景:异体血管移植之所以至今未能在临床应用,关键是异体组织抗原性排斥反应的难题未能得到解决。目的:制备动脉脱细胞血管基质,探讨脱细胞血管异体移植的可行性。方法:采用不同去垢剂(1%TritonX-100、1%SDS)多步骤对犬血管进行脱细胞处理,通过组织学和力学观测,建立犬动脉血管脱细胞的方法;并进行脱细胞血管的异体移植。结果与结论:经胰蛋白酶、低渗溶液和去垢剂TritonX-100、SDS等多步骤处理,犬颈总动脉血管的细胞基本脱除,细胞外基质保持完好,血管的弹性、韧性保存较好;用该法制备的犬颈总动脉(直径约4.0mm)进行异体移植,经10个月观察,4/5通畅。提示经去垢剂TritonX-100、SDS加低渗溶液、胰蛋白酶和蛋白酶抑制剂处理的多步法,可以脱除血管的细胞成分,细胞外基质和力学特性保持完好,是一种较好的方法;用该法制备的犬颈总动脉可以直接进行异体移植,是一可选择的血管移植材料。BACKGROUND：Up to now,allograft vascular transplantation has failed in clinical application,as antigenicity rejection of allogeneic tissues is unsolved.OBJECTIVE：To prepare acellular vascular matrix materials and to assess the feasibility of acellular vascular matrix materials used for allograft transplantation.METHODS：A five-step process,including hypotonic,hypertonic solutions,0.125% trypsogen,1% Triton X-100 and 1% SDS detergents,was performed in the carotid arteries from three dogs.Histological examination and general tensile tests were done on the samples.And the acellular matrix allografts were transplanted into another five dogs.RESULTS AND CONCLUSION：The five-step process could remove all cells with the extracellular matrix well retained.The acellular matrix allografts（diameter of about 4 mm） were patent at explanting up to 10 months in dogs.The acellular arterial graft matrix was well prepared with the five-step process including detergents,such as Triton X-100,SDS and trypsogen without compromising the graft structure or mechanical properties significantly.And the carotid artery allografts decellularized by this process can be used directly in allogeneic transplantation,which are ideal to vascular transplantation.

关 键 词：动脉血管 犬 脱细胞 同种异体移植 血管基质 

分 类 号：R318[医药卫生—生物医学工程]