不同细胞浓度种植于脱细胞血管基质上的细胞生长方式(英文)  

Growth patterns of cells with different implantation concentrations on the acellular vascular matrix

作　　者：池一凡[1] 许慧[2] 林明山[1] 侯文明[1] 牛兆倬[1] 孙勇[1] 孙忠东[1] 生伟[1] 孙龙[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院心脏外科,山东省青岛市266071 [2]青岛大学医学院附属医院麻醉科,山东省青岛市266071

出　　处：《中国组织工程研究》2012年第12期2153-2157,共5页Chinese Journal of Tissue Engineering Research

基　　金：the Scientific Research Foundation of Qingdao Municipal Science and Technology Commission,No.06-2-2-6-nsh~~

摘　　要：背景:对于组织工程血管而言,如何在平滑肌细胞层上成功获得致密的内皮细胞层是最为关键的。目的:探索不同细胞种植浓度对构建全生物化组织工程血管的影响。方法:先将不同浓度(5×105,5×107L-1)猪血管平滑肌细胞种植在猪脱细胞血管基质上,培养3d后再将不同浓度(5×105,5×107L-1)内皮祖细胞接种在平滑肌细胞-血管基质复合体上,构建片状全生物化组织工程材料。结果与结论:高浓度与低浓度平滑肌细胞在脱细胞血管基质上的细胞生长曲线相似,并且种植在孔板上和在脱细胞基质上的生长曲线亦相似,但低浓度组增殖较慢,覆盖率较低。细胞覆盖率由高到低的顺序为:高浓度内皮祖细胞+含高浓度平滑肌细胞的脱细胞基质>高浓度内皮祖细胞+含低浓度平滑肌细胞的脱细胞基质>低浓度内皮祖细胞+含高浓度平滑肌细胞的脱细胞基质>低浓度内皮祖细胞+含低浓度平滑肌细胞的脱细胞基质,且高浓度内皮祖细胞在脱细胞基质上可形成较为致密的细胞层,呈现出铺路石样生长方式。说明提高细胞接种浓度有利于其在材料表面快速形成致密的细胞层。BACKGROUND：How to successfully obtain compact endothelium layers on smooth muscle cells is the most crucial part for the tissue-engineered vessels.OBJECTIVE：To explore the effects of different cell implantation concentrations on the construction of the complete biological tissue-engineered blood vessels.METHODS：Different concentrations of porcine vascular smooth muscle cells（5×105,5×107 cells/L） were implanted on the porcine acellular vascular matrix to culture for 3 days.Then different concentrations of endothelial progenitor cells（5×105,5×107 cells/L） were implanted on the smooth muscle cell-vascular matrix composite to construct lamellar complete biological tissue-engineered blood vessels.RESULTS AND CONCLUSION：The growth curves of high concentrations of smooth muscle cells on the acellular vascular matrix were similar to that of low concentrations.Moreover,the growth curves of cells implanted in the culture plates were similar to that implanted on the acellular matrix.However,cells in the low concentration groups have relatively low proliferation activity and low coverage rate.The cell coverage rate decreased as follows：high concentrations of endothelial progenitor cells＋acellular matrix containing high concentrations of smooth muscle cells high concentrations of endothelial progenitor cells＋acellular matrix containing low concentrations of smooth muscle cells low concentrations of endothelial progenitor cells＋acellular matrix containing high concentrations of smooth muscle cells low concentrations of endothelial progenitor cells＋acellular matrix containing low concentrations of smooth muscle cells.Moreover,high concentrations of endothelial progenitor cells form relatively compact layers on the acellular matrix and show cobble-like growth.These findings indicate that an increase in the cell implantation concentrations is beneficial to the rapid formation of compact cell layers on the material surface.

关 键 词：组织工程血管 内皮祖细胞 平滑肌细胞 脱细胞血管基质 种植 

分 类 号：R318[医药卫生—生物医学工程]