心肌梗死模型大鼠心肌组织中白细胞介素17的表达  

Expression of interleukin-17 in the myocardium of rats with myocardial infarction

作　　者：陈昭喆[1,2] 陈志坚[1] 曾秋棠[1] 刘学刚[1] 张青[1] 刘立新[1] 朱云涛[1] 钱程[1] 杜以梅[1] 赵宁[1] 

机构地区：[1]华中科技大学协和医院心内科,华中科技大学同济医学院心血管病研究所,湖北省生物靶向治疗研究重点实验室,湖北省武汉市430022 [2]河南省信阳市中心医院心内科,河南省信阳市464000

出　　处：《中国组织工程研究》2012年第11期1985-1988,共4页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目。课题名称:TNF-α诱导急性缺血性室颤启动基质的作用及分子机制(30770880);TNF-α诱导Cx43异常分布在心梗后室性心律失常发生中的作用(30971242)~~

摘　　要：背景:白细胞介素17在多种心血管疾病中如自身免疫性心肌炎、病毒性心肌炎、扩张型心肌病、不稳定型冠心病等表达增加,在心血管病慢性炎症性过程起到了一定作用。目的:分析白细胞介素17在心肌梗死大鼠心肌中的表达特点。方法:采用液氮冷冻法建立大鼠心肌梗死模型,将造模成功的66只大鼠按照不同的观察时间随机摸球法均分为1,3,7,14,28,56d组,另设对照组8只。采用酶联免疫吸附测定法、免疫荧光激光共聚焦技术及蛋白免疫印迹法检测白细胞介素17的表达。结果与结论:造模成功率为73%。大鼠心肌梗死的发生发展过程中,白细胞介素17在心肌组织大量表达,且随着时间变化而逐渐升高,在28d时表达最为显著。说明白细胞介素17作为一种强力的促炎症因子在心肌梗死大鼠心肌组织中大量表达,有可能与心肌梗死后的心室重塑及心律失常的发生有关。BACKGROUND： Expression of interleukin-17 increases in many cardiovascular disease such as autoimmune myocarditis, viral myocarditis, dilated cardiomyopathy and unstable coronary artery disease. Interleukin-17 plays an important role in chronic inflammatory process of cardiovascular diseases. OBJECTIVE： To study the expression of interleukin-17 in the myocardium after myocardial infarction in rats. METHODS： Liquid nitrogen freezing method was used to establish a rat myocardial infarction model. Totally 66 rats with modeling successful were randomly divided into six groups according to different observation times： 1, 3, 7, 14, 28, 56 days groups. And eight rats were selected as controls. The expression of interleukin-17 was examined by enzyme linked immunosorbent assay, laser confocal scanning microscope and Western blot. RESULTS AND CONCLUSION： Modeling success rate was 73%. With the development of myocardial infarction, interleukin-17 was abundantly expressed in the myocardial tissue and gradually increased over time. The expression of interleukin-17 in the 28 days group increased most significantly. Interleukin-17 as a potent pro-inflammatory factor can strongly express in rat myocardium after myocardial infarction, which may be related to the incidence of ventricular remodeling and arrhythmia.

关 键 词：白细胞介素17 心肌梗死 大鼠 模型 心肌组织 

分 类 号：R318[医药卫生—生物医学工程]