青老年大鼠脑缺血模型的比较  

Young rat model versus old rat model for cerebral ischemia

作　　者：刘宗涛[1] 秦永生[1] 刘江[2] 刘远新[3] 姜涛[4] 

机构地区：[1]新疆医科大学,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆医科大学附属中医医院VIP病房,新疆维吾尔自治区乌鲁木齐市830000 [3]新疆医科大学附属中医医院神经内科,新疆维吾尔自治区乌鲁木齐市830000 [4]新疆医科大学第一附属医院实验动物研究中心,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国组织工程研究》2012年第11期2003-2006,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:大鼠具有成本低,种系内纯合性好,脑血管解剖特性与人类相似等特点,是目前脑缺血研究最常用的实验动物。目的:观察青老年大鼠大脑中动脉梗死后的行为学变化,分析年龄对脑缺血的影响。方法:将青、老年SD大鼠随机分为青、老年假手术组,青、老年模型组(同侧颈总动脉永久结扎大脑中动脉线栓法制备大脑中动脉梗死模型)4组。结果与结论:老年假手术组体质量低于青年假手术组(P<0.05),但高于老年模型组(P<0.05),老年模型组体质量低于青年模型组(P<0.05)。术后第1,3,5,7天老年模型组改良神经功能损害程度评分高于青年模型组(P<0.05);与青年模型组及青、老年假手术组比较,术后第3,8,12周老年模型组逃避潜伏期明显延长,跨过平台所在位置的次数明显减少(P<0.05)。表明在同等缺血打击下,老龄鼠脑缺血模型缺血损伤重、修复能力低,其神经功能恢复、学习和记忆能力明显逊于青年大鼠,提示增龄因素是研究脑缺血后神经损伤的重要影响因素之一。BACKGROUND： At present, rats are the most commonly used experimental animals in cerebral ischemia research. Rats have many advantages, such as inexpensive, good intraspecific homozygosity and similar cerebrovascular anatomical characteristics with human beings. OBJECTIVE： To explore the influence of aging to cerebral ischemia by observing the behavior changes in young and old rats with middle cerebral artery occlusion. METHODS： Young and old Sprague Dawley rats were randomly divided into four groups： young sham-operation group, old sham-operation group, young model group and old model group. Middle cerebral artery occlusion model was established by permanent ligation of the ipsilateral common carotid artery using the intraluminal suture technique. RESULTS AND CONCLUSION： The body mass index of the old sham-operation group was significantly lower than that of the young sham-operation group （P 0.05）, but higher than that of the old model group （P 0.05）. The body mass index of the old model group was lower than that of the young model group （P 0.05）. The modified neurological severity score of the old model group was significantly higher than that of the young model group on the 1st, 3rd, 5th and 7th days after operation （P 0.05）. The escape latency of the old model group was significantly longer than those of the other groups at the 3rd, 8th and 12th weeks after operation. The number of crossing platform location in the old model group was significantly less than those in the other groups （P 0.05, respectively）. These findings demonstrated that compared with the young rat cerebral ischemia model with the same ischemia state, the old rat cerebral ischemia model showed more severe ischemia damage, lower repair capacity, and the neural functional recovery, spatial learning and memory ability was obvious inferior to that of the young rat model. It indicates that aging is one of the important factors that influence the neural injury after cerebral ischemia.

关 键 词：大脑中动脉梗死 动物模型 改良神经损害程度评分 学习 记忆 组织构建实验造模 

分 类 号：R318[医药卫生—生物医学工程]