Spatio-temporal Expression Study of Phosphorylated 70-kDa Ribosomal S6 Kinase (p70S6k) in Mesial Temporal Lobe Epilepsy  

作　　者：Xiao-liang Xing Long-ze Sha Yuan Yao Yan Shen Li-wen Wu Qi Xu 

机构地区：[1]National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100005, China [2]Department of Neurology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100730, China

出　　处：《Chinese Medical Sciences Journal》2012年第1期7-10,共4页中国医学科学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China (31021091, 30971001);Beijing Natural Science Foundation (7102109);Fok Ying Tong Education Foundation (121024)

摘　　要：Objective To determine the spatio-temporal expression of p70S6k activation in hippocampus in mesial temporal lobe epilepsy. Methods Temporal lobe epilepsy model was established by stereotaxically unilateral and intrahip-pocampal injection of kainite acid (KA) in adult male C57BL/6 mice. Latent and chronic epileptogenesis were represented by mice 5 days after KA injection (n=5) and mice 5 weeks after KA injection (n=8), respectively. Control mice (n=5) were injected with saline. Immunohistochemical assays were performed on brain sections of the mice. Results Hippocampus both ipsilateral and contralateral to the KA injection displayed significantly up-regulated pS6 immunoreactivity in dispersed granule cells in 5-day and 5-week model mice. Conclusion The activation of p70S6k is mainly located in the dentate gyrus in KA-induced mouse model of temporal lobe epilepsy, indicating that the activation may be related with the disperse degree and hypertrophy of granule cells.

关 键 词：mesial temporal lobe epilepsy P70S6K kainate acid IMMUNOREACTIVITY 

分 类 号：Q426[生物学—神经生物学] S965.334[生物学—生理学]