MiR-615在平行培养的神经干细胞与运动神经元的表达比较  

MiR-615 Expressions in Parallelly Isolated Neural Stem Cells And Motor Neurons

作　　者：索耀君[1] 王春芳[2] 

机构地区：[1]浙江大学医学院附属儿童医院麻醉科,杭州市310003 [2]山西医科大学实验动物中心,太原市030001

出　　处：《中国组织化学与细胞化学杂志》2012年第2期152-156,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的探讨MiR-615在脊髓源性神经干细胞与运动神经元间的表达特征。方法通过免疫磁珠法分离纯化胚胎大鼠脊髓运动神经元;通过神经克隆球形成技术分离纯化胚胎大鼠脊髓源性神经干细胞。采用TaqMan miR-615Assay定量检测培养的脊髓源性神经干细胞与运动神经元中miR-615的表达差异。结果通过平行培养技术分别获得了纯化的脊髓源性神经干细胞与运动神经元。定量检测结果显示,miR-615在分离的运动神经元中较在脊髓源性神经干细胞中显著高表达。结论本文提示miR-615可能在神经干细胞定向分化为运动神经元过程中发挥重要的调节作用。Objective To explore the expression of miR-615 in parallelly cultured neural stem cells（NSCs） and motor neurons（MNs）. Methods A parallel isolation and purification of MNs and NSCs from the same rat embryonic spinal cord sample was performed.A method allowing for the isolation of highly purified rat MNs was developed using immunomagnetic technology.The isolation of NSCs was performed through the classical neurosphere formation procedure.The miR-615 expression was quantified using the TaqMan miR-615 Assay. Results The purified MNs extended neurites and showed a typical MN morphology.The NSCs formed multi-cellular neurospheres.The level of miR-615 expression in MNs was significantly higher than that in NSCs. Conclusion These results suggest that miR-615 can regulate the directed differentiation of NSCs into MNs.

关 键 词：miR-615 神经干细胞 运动神经元 调节 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]