CO_2气腹对大鼠胃肠动力和十二指肠组织一氧化氮、丙二醛含量的影响  

Influence of CO_2 pneumoperitoneum on gastrointestinal motility and changes of nitric oxide and malondialdehyde in rat duodenum

作　　者：牟峰[1] 孟凌新[2] 李丹[2] 

机构地区：[1]沈阳市第六人民医院,辽宁沈阳110006 [2]中国医科大学盛京医院

出　　处：《腹腔镜外科杂志》2012年第4期256-258,共3页Journal of Laparoscopic Surgery

摘　　要：目的:建立大鼠CO2气腹模型,观察不同气腹压力对大鼠胃肠动力指标及十二指肠组织一氧化氮、丙二醛含量的影响。方法:随机将36只大鼠按不同气腹压力(0 mmHg,10 mmHg,15 mmHg)分为三组,每组均接受2 h气腹时程的处理,观察不同气腹压力下胃排空率及小肠推进比的变化趋势,以及十二指肠组织一氧化氮、丙二醛水平的变化。结果:气腹2 h时,低压组(10 mmHg)较气腹前胃残留率显著升高,小肠推进比显著下降(P<0.05),一氧化氮下降非常明显(P<0.05),丙二醛有升高趋势,但差异无统计学意义(P>0.05);高压组(15 mmHg)各指标变化更为显著(P<0.01)。结论:CO2气腹使胃残留率增加,小肠推进比下降,十二指肠一氧化氮含量减低,丙二醛升高,随着气腹压力的增高,此作用显著增强。] Objective：To discuss the influence of CO2 pneumoperitoneum on gastrointestinal motility and changes of nitric ox- ide （NO） and malondialdehyde （MDA） in duodenum of rats. Methods：Thirty-six male rats were randomly divided into three groups according to different pneumoperitoneum pressure （0 mmHg, 10 mmHg, 15 mmHg） and each group accepted 2 hours of pneumoperitoneum management. Food residue rate of stomach, food advancement in small intestine, NO and MDA in duodenum were measured. Resuits：Compared with rats in control group （0 mmHg）, after 2 h of pneumoperitoneum, rats in low-pressure group（ 10 mmHg） had more food residue in stomach （ P 〈 0.05 ） and lower food advancement in small intestine （ P 〈 0.05 ）. NO decreased significantly in low-pres- sure group （ P 〈 0.05 ） and there was no significant difference in MDA between low-pressure group and control group（ P 〉 0.05 ） ,while rats in high-pressure group showed higher MDA（P 〈 O. 01 ）. Conclusions：CO2 pneumoperitoneum increases food residue rate of stom- ach, decreases food advancement in small intestine, decreases NO and increases MDA in duodenum. As the elevation of pneumoperitone- um pressure, this influence is more obvious.

关 键 词：气腹 胃肠活动 一氧化氮 丙二醛 

分 类 号：R656[医药卫生—外科学]