Prognostic significance of PTEN,Ki-67 and CD44s expression patterns in gastrointestinal stromal tumors  

作　　者：Yu-Mei Liang Xiang-Hong Li Wen-Mei Li You-Yong Lu 

机构地区：[1]Department of Pathology,Chinese PLA the 309th Hospital,Beijing 100091,China [2]Department of Pathology,Chinese PLA General Hospital,Beijing 100853,China [3]Laboratory of Molecular Oncology,Peking University School of Oncology,Beijing Cancer Hospital/Institute,Beijing 100036,China

出　　处：《World Journal of Gastroenterology》2012年第14期1664-1671,共8页世界胃肠病学杂志（英文版）

基　　金：Supported by Grants from the National Key Basic Research Program Project of China,No.2004CB518708;National BioTech 863 program,No. 2002-BA711 A11

摘　　要：AIM:To develop a prognostic approach for gastrointestinal stromal tumors(GISTs) using a cluster of indicators and follow-up information.METHODS:One hundred and four GISTs that had not been subjected to targeted therapies were collected and classified by NIH risk assessment and anatomic location.By immunohistochemistry,the expressions of PTEN,Ki-67,CD44s matrix metalloproteinase(MMP)-9 and TIMP-1 were detected on tissue microarray.Univariate and multimarker survival analyses were performed and then a COX hazard proportion model was constructed to evaluate a cluster of predictors of GIST.RESULTS:Our data showed small intestinal GIST are more aggressive than gastric GIST.The NIH risk assessment correlated with disease-free survival foreither gastric GIST or small intestinal GIST.Immunohistochemical analysis revealed that Ki-67 labeling indexes(LIs) < 5% predicted higher disease-specific survival(DSS) in gastric and small intestinal GIST.CD44s positivity and PTEN LIs ≥ 50% correlated with higher DSS in gastric GIST.MMP-9 and TIMP-1 had no correlation with survival.Multimarker analysis revealed that the expression pattern of PTEN LIs ≥ 50% combined with Ki-67 LIs < 5% and CD44s positivity reliably predicted favorable outcomes for gastric GIST(P = 0.009),as did the combination of PTEN LIs ≥ 50% and Ki-67 LIs < 5% for small intestinal GIST(P = 0.011).Authors also found that high NIH risk grade was correlated with DSS in patients with gastric GIST and disease-free survival in patients with small intestinal GIST.CONCLUSION:PTEN LIs ≥ 50%,Ki-67 LIs < 5% and CD44s positivity provides an accurate,favorable prognosis for gastric GIST.PTEN LIs ≥ 50% and Ki-67 LIs < 5% does the same for small intestinal GIST.Ki-67 LIs enhances the NIH assessment.AIM：To develop a prognostic approach for gastrointestinal stromal tumors（GISTs） using a cluster of indicators and follow-up information.METHODS：One hundred and four GISTs that had not been subjected to targeted therapies were collected and classified by NIH risk assessment and anatomic location.By immunohistochemistry,the expressions of PTEN,Ki-67,CD44s matrix metalloproteinase（MMP）-9 and TIMP-1 were detected on tissue microarray.Univariate and multimarker survival analyses were performed and then a COX hazard proportion model was constructed to evaluate a cluster of predictors of GIST.RESULTS：Our data showed small intestinal GIST are more aggressive than gastric GIST.The NIH risk assessment correlated with disease-free survival foreither gastric GIST or small intestinal GIST.Immunohistochemical analysis revealed that Ki-67 labeling indexes（LIs） 5% predicted higher disease-specific survival（DSS） in gastric and small intestinal GIST.CD44s positivity and PTEN LIs ≥ 50% correlated with higher DSS in gastric GIST.MMP-9 and TIMP-1 had no correlation with survival.Multimarker analysis revealed that the expression pattern of PTEN LIs ≥ 50% combined with Ki-67 LIs 5% and CD44s positivity reliably predicted favorable outcomes for gastric GIST（P = 0.009）,as did the combination of PTEN LIs ≥ 50% and Ki-67 LIs 5% for small intestinal GIST（P = 0.011）.Authors also found that high NIH risk grade was correlated with DSS in patients with gastric GIST and disease-free survival in patients with small intestinal GIST.CONCLUSION：PTEN LIs ≥ 50%,Ki-67 LIs 5% and CD44s positivity provides an accurate,favorable prognosis for gastric GIST.PTEN LIs ≥ 50% and Ki-67 LIs 5% does the same for small intestinal GIST.Ki-67 LIs enhances the NIH assessment.

关 键 词：Gastrointestinal stromal tumor PROGNOSIS PTEN KI-67 CD44S 

分 类 号：Q253[生物学—细胞生物学] S852.2[农业科学—基础兽医学]