转基因玉米花期抗旱性指标与综合评价  

Drought Resistance Indexes at Flowering Stage and Compressive Evaluations in Transgenic Maize

作　　者：贾俊香[1] 贾炜珑[2] 

机构地区：[1]山西农业大学资源环境学院,山西太谷030801 [2]山西省农业科学院旱地农业研究中心,山西太原030006

出　　处：《山西农业科学》2012年第4期326-328,共3页Journal of Shanxi Agricultural Sciences

基　　金：山西省科技攻关项目(021006)

摘　　要：采用盆栽法,以非转基因玉米为对照,在水分胁迫下,测定转干旱诱导表达启动子驱动的海藻糖合酶基因(RT)、果聚糖合酶基因(PK)、Ubiquitin启动子驱动的海藻糖合酶基因(H)与甜菜碱醛脱氢酶基因(BC)的玉米植株花期的相对含水量、保水力、叶水势、相对电导率、光合速率与蒸腾速率指标;并采用模糊隶属度对4类转基因材料进行抗旱性综合评价。结果表明,在玉米花期,所选指标均可用来筛选玉米的抗旱性能;转基因材料的抗旱性强于非转基因材料;4类转基因材料的抗旱强度为RT最强,PK与BC居中,H最弱。Non-transgenic maize was used as the control substance, a pot experiment was conducted to study the drought-resistance of transgenic maize including trehalose synthase gene driven by drought induce （RT）, fructosan synthase gene （PK）, trehalose synthase gene driven by Ubiquitin （H）, betaine aldehyde dehydrogenase （BC）. Relative water contents, water-holding capacity, relative conductivity rate, photosynthesis rate and transpiration rate were all determined at maize flowering stage under water stress. Fuzzy membership was used to evaluate comprehensively the drought-resistance of four transgenic materials. The results showed that indexes selected were all used to estimate drought-resistance of maize. Drought-resistance of transgenic maize was greater than that of the no-transgeuic ones. Drought resistance from RT was the strongest, that from BC and PK neatly equal, and H the weakest.

关 键 词：转基因玉米 花期 抗旱性 评价 

分 类 号：S513.01[农业科学—作物学]