白芷提取分离物体外对酪氨酸酶的抑制作用  

Inhibition of Extracts from Angelica Dahurica on Tyrosinase in vitro

作　　者：胡大强[1] 侯丽娜[2] 傅若秋[1] 孟德胜[1] 宋宏宇[1] 向明凤[1] 徐靖[1] 

机构地区：[1]第三军医大学第三附属医院药剂科,重庆400042 [2]蚌埠医学院药学系

出　　处：《中国药师》2012年第4期457-459,共3页China Pharmacist

摘　　要：目的:体外评价白芷提取分离物对酪氨酸酶的抑制作用。方法:白芷提取分离物采用薄层色谱法鉴别,HPLC法测定分离物中欧前胡素含量,多巴速率氧化法测定其对酪氨酸酶的抑制率。结果:白芷提取分离物主要为欧前胡素,含欧前胡素0.01,0.05,0.10μg.ml-1分离物对酪氨酸酶的抑制率分别为(15.92±5.3)%、(25.34±3.94)%、(40.38±1.45)%。结论:白芷提取分离物对酪氨酸酶活性具有抑制作用。Objective： To study the inhibitory effect of the extracts from angelica dahurica on tyrosinase in vitro.Method： The angelica dahurica extracts isolated by macroporous resin were identified by TLC,the content of imperatorin in the extracts was determined by HPLC,and the inhibition rate of angelica dahurica extracts on tyrosinase in vitro was studied by the oxidation rate of L-dopa.Result： The major component of the angelica dahurica extracts was imperatorin.The inhibition rate on tyrosinase were 15.92%±5.3%,25.34%±3.94% and 40.38%±1.45% for different imperatorin concentrations（0.01,0.05 and 0.10 μg·ml-1）.Conclusion： The angelica dahurica extracts show the capability of tyrosinase inhibition.

关 键 词：白芷 欧前胡素 酪氨酸酶 高效液相色谱法 

分 类 号：R285.5[医药卫生—中药学]