K^+及主要离子间交互作用对凡纳滨对虾存活、生长及体内ATP酶的影响  

Effect of K^+ and the interaction of major ions on survival,growth and ATP enzymes specific activity of Litopenaeus vannamei

作　　者：张立田[1] 戴习林[1] 臧维玲[1] 邓平平[1] 邹卫丽[1] 丁福江 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海申漕特种水产开发公司,上海201376

出　　处：《广东农业科学》2012年第6期116-120,共5页Guangdong Agricultural Sciences

基　　金：上海市科技兴农重点攻关项目(沪农科攻字[2010]第1-6号);上海市科技兴农科技推广项目(沪农科推字[2008]第5-1号);上海市教育委员会重点学科建设项目(J50701)

摘　　要：采取L16(215)正交设计试验,开展5周凡纳滨对虾养殖试验,分析养殖水体中K+及K+、盐度、Ca2+、Mg2+四因子间交互作用对凡纳滨对虾存活、生长及体内ATP酶的影响。结果表明:K+对凡纳滨对虾成活率、体长和体重增加及体内ATP酶活性均具有显著影响,K+浓度为150 mg/L时成活率最高,而K+浓度为50 mg/L时生长速度最快,Na+-K+-ATPase、Mg2+-ATPase、Ca2+-ATPase酶活也最高;K+、盐度、Ca2+、Mg2+四因子间交互作用对凡纳滨对虾存活、生长及体内ATP酶也具有显著影响。本试验因素水平组合中凡纳滨对虾成活率的最佳组合为盐度15、K+150 mg/L、Ca2+100 mg/L、Mg2+300 mg/L,生长速度的最佳组合为盐度15、K+50 mg/L、Ca2+100 mg/L、Mg2+100 mg/L,凡纳滨对虾体内Na+-K+-ATPase、Mg2+-ATPase、Ca2+-ATPase酶活的最佳组合为盐度5、K+50 mg/L、Ca2+100 mg/L、Mg2+100 mg/L。In order to analysis the role of K^＋ and the interaction of salinity,Ca^2＋,Mg^2＋,K^＋ in water to the survival,growth and ATP enzymes specific activity of Litopenaeus vannamei,the survival rate,daily length gain,daily weight gain and ATP enzymes specific activity were compared during the 5 weeks breeding cycle by L16（215） orthogonal experimental design.Results showed that K^＋ had significant effect on survival rate,length and weight gain,ATP enzymes specific activity in Litopenaeus vannamei.The highest survival rate at K^＋ of 150 mg/L,the fastest growth rate and optimal ATP enzymes activity at K^＋ of 50 mg/L.And the results showed the interaction of salinity,Ca^2＋,Mg^2＋,K^＋ in water also had significant effect on survival rate,length and weight gain,ATP enzymes specific activity in Litopenaeus vannamei.The best combination of survival rate in the experiment was salinity of 15,K^＋ of 150 mg/L,Ca^2＋ of 100 mg/L,Mg^2＋ of 300 mg/L,the best combination of growth rate was salinity of 15,K^＋ of 50 mg/L,Ca^2＋100 mg/L,Mg^2＋100 mg/L,the best combination of Na＋-K^＋-ATPase,Mg^2＋-ATPase,Ca^2＋-ATPase specific activity in Litopenaeus vannamei was salinity of 5,K^＋ of 50 mg/L,Ca^2＋ of 100 mg/L,Mg^2＋ of 100 mg/L.

关 键 词：凡纳滨对虾 K+ 交互作用 存活 生长 ATP酶 

分 类 号：S966.1[农业科学—水产养殖]