生长因子对兔骨髓间充质干细胞增殖的影响  

Proliferative Effects of Growth Factors on Bone Marrow Mesenchymal Stem Cells of Rabbits in vitro

作　　者：和小娥[1] 任卫青[1] 邓立新[1] 何俊丹[1] 秦佳晨[1] 吕婧玉[1] 曹桂彬[1] 王新庄[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002

出　　处：《畜牧兽医学报》2012年第4期553-557,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河南省基础与前沿技术研究计划(092300410081);现代农业产业技术体系专项资金(30300324)

摘　　要：旨在研究碱性成纤维细胞生长因子(bFGF)对兔骨髓间充质干细胞(BMSCs)体外生长及增殖的影响。体外培养并鉴定兔BMSCs,用不同浓度的bFGF(5、10、20、40、80和100μg.L-1)作用于兔BMSCs,用四甲基偶氮唑盐比色法(MTT)、流式细胞仪观察细胞的生长及增殖情况。结果,经免疫细胞化学法和分化反推法鉴定所分离培养的细胞为兔骨髓间充质干细胞;MTT法结果显示,bFGF浓度为80μg.L-1时细胞的增殖能力最强(P<0.01),在培养第3天即出现显著的促增殖作用(P<0.01);流式细胞仪结果显示,bFGF组BMSCs的增殖指数明显高于对照组(P<0.01)。说明bFGF有促进体外培养兔BMSCs增殖的作用,浓度为80μg.L-1时促增殖能力最强,可以作为体外扩增培养兔骨髓间充质干细胞的最佳培养条件。To study the effects of basic fibroblast growth factor（bFGF） on growth and proliferation of rabbit bone marrow mesenchymal stem cells（BMSCs） in vitro,rabbit BMSCs were cultured and identified in vivo,treated with bFGF at final concentration of 5,10,20,40,80,100 μg·L-1.Cell growth and proliferation were detected by MTT assay and flow cytometer.MTT assay showed that proliferation promotion effect of bFGF nearly reached the peak at 80 μg·L-1（P〈0.01）.Compared with the negative control group,significant proliferation promotion effect was detected in the bFGF groups from 72 h（P〈0.01）;Flow cytometer result showed that proliferation index of bFGF groups BMSCs was higher than that of control group（P〈0.01）.Basic fibroblast growth factor can promote proliferation of rabbit BMSCs in vitro,80 μg·L^-1 is the optimum concentration for in vitro culture which can be used as the best culture condition of rabbit BMSCs proliferation in vitro.

关 键 词：兔 骨髓间充质干细胞 碱性成纤维细胞生长因子(bFGF) 体外增殖 

分 类 号：S829.1[农业科学—畜牧学] Q343[农业科学—畜牧兽医]