猪圆环病毒2型感染对猪瘟弱毒疫苗接种猪免疫应答的影响  

Influence of Porcine Circovirus Type 2 Infection on Immune Response of Pigs to Classical Swine Fever Vaccine

作　　者：赵丹萍[1] 陶彬[1] 陈立功[1] 李艳琴[1] 宋勤叶[1] 李潭清[1] 杨芳[1] 李红园[1] 袁洪兴[1] 

机构地区：[1]河北农业大学农业部动物疫病病原生物学华北区观测实验站,保定071000

出　　处：《畜牧兽医学报》2012年第4期580-587,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河北省自然基金项目(C2010000673);国家自然基金项目(30771600);河北农业大学大学生科技创新活动基金

摘　　要：为探索猪圆环病毒2型(PCV2)感染对猪瘟(CSF)弱毒疫苗接种猪免疫应答的影响,将20头28d断奶仔猪随机分为V-I、I-V、V和C 4组,5头.组-1。V-I组在接种CSF弱毒疫苗后2d感染PCV2;I-V组在感染PCV2后2d接种CSF弱毒疫苗;V组只接种CSF弱毒疫苗;C组为空白对照组。共免疫2次,间隔21d。免疫后定期检测血清CSFV特异性抗体水平、外周血淋巴细胞(PBLC)增殖活性和PBLC中IFN-γ、IL-2、IL-4和IL-10mRNA的表达水平。结果显示:在CSF弱毒疫苗免疫前或免疫后感染PCV2,均会导致CSFV抗体水平低下,血清抗体阳转率下降,PBLC增殖活性降低。初次免疫后,V-I与I-V组的PBLC内IFN-γ、IL-2、IL-4和IL-10mRNA的表达量严重不足,其中V-I组的表达量最低。加强免疫后V-I与I-V组的PBLC内IFN-γ与IL-10的表达失衡,IL-2和IL-4的表达缺乏,其中I-V组的细胞因子表达失衡和缺乏更严重。上述研究表明,CSF弱毒疫苗免疫前或免疫后感染PCV2均会影响机体的体液和细胞免疫应答水平,导致PBLC内细胞因子的表达严重抑制和紊乱。To study the influence of porcine circovirus type 2（PCV2） infection on immune response of pig to classical swine fever（CSF） vaccine,twenty 28-day-old healthy weaning piglets were randomly divided into four groups of five（Group V-I,I-V,V and C）.The piglets in Group V-I were intramuscularly injected with CSF vaccine two days before being inoculated with PCV2,piglets in Group I-V were infected by PCV2 two days before being injected with CSF vaccine,piglets in Group V were only vaccinated by CSF vaccine and Group C as the blank control without inoculating anything.Piglets were vaccinated twice at an interval of twenty-one days and blood samples were collected periodically so as to detect CSFV-specific serum antibody,lymphocyte proliferation activity and mRNA expression levels of IFN-γ,IL-2 and IL-4 in peripheral blood lymphocyte（PBLC）.PCV2 infection,either before or after vaccination,reduced CSFV-specific serum antibody production,seroconversion and lymphocyte proliferation activity.After the primary vaccination,expression of IFN-γ,IL-2,IL-4 and IL-10 mRNA decreased seriously in PBLC from piglets in Group V-I and Group I-V,especially in Group V-I.Following the secondary vaccination,expressions of IFN-γ and IL-10 mRNA in PBLC from Group V-I and Group I-V appeared seriously unbalanced and those of IL-2 as well as IL-4 mRNA were deficient,especially in Group I-V.In conclusion,PCV2 infection before or after vaccination with CSF vaccine affected porcine humoral and cellular immunity and led to expression suppression and unbalance of cytokines in PBLC.

关 键 词：PCV2 猪瘟弱毒疫苗 抗体 淋巴细胞增殖活性 细胞因子 

分 类 号：S852.659.2[农业科学—基础兽医学] S852.651[农业科学—兽医学]