视黄醇对LPS诱发的原代培养大鼠乳腺上皮细胞炎症反应的调节  

Modulation of Retinoic Acid in LPS-induced Inflammation in Primary Culture of Rat Mammary Epithelial Cells

作　　者：顾蓓蓓[1,2] 苗晋锋[1] 卢劲晔[1] 邹思湘[1] 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]泰州出入境检验检疫局,泰州225300

出　　处：《畜牧兽医学报》2012年第4期627-633,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30671533;31072104)

摘　　要：本研究旨在优化大鼠乳腺上皮细胞培养体系,建立LPS诱发的炎症反应模型,研究视黄醇对其炎症反应的调节机制。采用胶原酶与透明质酸酶联合消化分离大鼠乳腺上皮细胞。待细胞铺满整个培养瓶的90%时,(1)用不同浓度的LPS处理乳腺上皮细胞,24h收集细胞及培养上清液;(2)分为试验组(添加1μmol.L-1视黄醇)和对照组,处理24h后更换培养液,用10μg.mL-1的LPS处理细胞,分别于不同时间点收集细胞。结果表明:LPS处理大鼠乳腺上皮细胞后引起各个时间点炎性因子mRNA表达极显著升高,视黄醇能显著下调上述炎性细胞因子的表达。视黄醇预处理能减轻LPS诱发的大鼠乳腺上皮细胞的炎症损伤。Lipopolysaccharide（LPS） induced primary culture of rat mammary epithelial cells（rMEC） inflammatory model was established and the protective mechanism of retinoic acid was studied.rMEC s were separated through digestion by collagenase and hyaluronidase.When cells were grown to 90% confluence in 6-well plates,（1） cells were treated with different concentrations of LPS;（2） cells were treated with 1 μmol·L^-1 retinoic acid before stimulation with 10 μg· mL^-1 LPS.Cells and supernatants were collected.In this study,inflammatory models of primary culture of rMEC were successfully established.Stimulation of rMEC with LPS elicited a marked increase in mRNA expression for inflammatory mediators.Treatment of retinoic acid significantly decreased the mRNA expression of those mediators.Retinoic acid could reduce LPS-induced inflammatory reaction.These results further indicate that retinoic acid may protect mastitis from pro-inflammatory cytokine-mediated damage of mammary epithelial cells.

关 键 词：脂多糖 乳腺上皮细胞 视黄醇 炎性反应 

分 类 号：S852.2[农业科学—基础兽医学]