不同阴离子对二色补血草盐腺Na^+分泌速率的影响  

Effects of Different Anions on Na^+ Secretion Rate of Salt Glands in the Leaves of Limonium bicolor

作　　者：杨剑超[1] 丁烽[1,2] 吴蕊蕊[1] 袁芳[1] 王宝山[1] 

机构地区：[1]山东师范大学生命科学学院逆境植物重点实验室,济南250014 [2]山东轻工业学院食品与生物工程学院,济南250355

出　　处：《植物生理学报》2012年第4期397-402,共6页Plant Physiology Journal

基　　金：国家自然科学基金(30870138;31070158);国家支撑计划项目(2009BADA7B05)

摘　　要：本文以二色补血草(Limonium bicolor)为实验材料,用Hoagland营养液和200mmol·L-1NaCl、NaBr、NaNO3溶液分别处理12h,测定二色补血草盐腺的Na+分泌速率、叶片Na+含量和MDA(丙二醛)含量以及质膜透性,并利用非损伤微测技术探索可能与盐腺相关的转运蛋白,以探讨不同阴离子对二色补血草盐腺分泌Na+的作用及其可能原因。结果表明:在NaCl处理12h时二色补血草叶片Na+分泌速率达到最大,然后逐渐下降;不同钠盐处理下叶片Na+分泌速率为NaCl>NaBr=NaNO3>Hoagland,而叶片Na+含量为NaBr>NaCl>NaNO3>Hoagland;不同盐处理下叶片质膜透性和MDA含量无显著性差异;利用Na-K-Cl共转运体专一性抑制剂bumetanide处理发现Na+分泌速率显著降低。这些结果表明Na-K-Cl共转运体可能参与盐腺分泌Na+。Exo-recretohalophyte Limonium bicolor was treated with Hoagland＇s solution （control）, 200 mmol.L-1 NaCl, NaBr and NaNO3 for 12 h, respectively. In order to examine the effects of different anions on Na＋ secretion ability and to elucidate possible reasons, the salt secretion rate of salt glands, Na＋ content, MDA content, and the plasma membrane permeability were determined. Plasmalemma transporters such as Na-K-Cl co-transporter was investigated using the Scanning Ion-selective Electrode Technique （SIET）. The results showed that Na＋ secretion rates of salt glands in Limonium bicolor leaves significantly increased with time and reached the highest values at 12 h and then gradually declined. Na＋ secretion rates of salt glands in Limonium bicolor leaves under different salt treatments were NaCl〉NaBr=NaNO3〉Hoagland, but Na＋ contents in leaves were NaBr〉NaCl〉NaNO3〉Hoagland. Moreover, there were no significant differences in leaf plasma membrane damage and MDA contents among different treatments. The Na＋ secretion rate significantly reduced by treating salt glands with Na-K-Cl co-transporter inhibitor bumetanide. These results suggested that the Na-K-Cl cotransporter is possibly involved in the process of Na＋ secretion of salt glands.

关 键 词：阴离子 二色补血草 盐腺 Na+分泌 非损伤微测技术 

分 类 号：Q945[生物学—植物学]