少腹逐瘀丸对子宫内膜异位症大鼠MMP-9和TIMP-1 mRNA表达的影响  

Effect of Shaofu Zhuyu Pill on expression of MMP-9 and TIMP-1 mRNA in endometriosis rats

作　　者：刘姣[1] 贺克[2] 李清[1] 

机构地区：[1]河北医科大学中医学院,河北石家庄050091 [2]石家庄市第四医院,河北石家庄050011

出　　处：《中成药》2012年第4期610-613,共4页Chinese Traditional Patent Medicine

基　　金：河北省中医药管理局科研计划项目(2011004)

摘　　要：目的探讨少腹逐瘀丸(当归、赤芍、川芎等)对患子宫内膜异位症大鼠子宫内膜组织中基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶抑制剂-1(TIMP-1)表达的影响。方法采用自体移植法建立子宫内膜异位症大鼠模型,将造模成功的大鼠随机分为模型组、孕三烯酮组、少腹逐瘀丸高剂量组、低剂量组,另设假手术组。给药30 d后,以RT-PCR法检测大鼠在位以及异位内膜组织中MMP-9和TIMP-1 mRNA表达。结果少腹逐瘀丸能显著降低子宫内膜异位症大鼠MMP-9 mRNA的表达(P<0.01),显著升高TIMP-1 mRNA的表达(P<0.01)。结论少腹逐瘀丸可调节MMP-9/TIMP-1的平衡,这可能是其治疗子宫内膜异位症的作用机制之一。AIM To study the effect of Shaofu Zhuyu Pill （Angelicae sinensis Radix, Paeoniae Radix rubra, Chuanxiong Rhizoma, etc. ）（SZP） on the expression level of MMP-9 and TIMP-1 mRNA in endometriosis （EMS） rats. METHODS The model of EMS rats was made by implanted tunica intima method. Then forty rats were ran- domly divided into five groups, sham operation group, EMS model group, gestrinone group, high dose SZP group and low dose SZP group. After consecutive intragastric administration for 30 days, the expression levels of MMP-9 and TIMP-1 mRNA were detected by RT-PCR. RESULTS SZP could significantly down-regulate the expression level of MMP-9 mRNA in EMS rats （ P 〈 0.01 ） , and significantly up-regulate the expression level of TIMP-1 mRNA （P 〈0.01）. CONCLUSION SZP could treat EMS through adjusting the balance of MMP-9 and TIMP-1.

关 键 词：少腹逐瘀丸 子宫内膜异位症 基质金属蛋白酶-9 基质金属蛋白酶抑制剂-1 大鼠 

分 类 号：R285.5[医药卫生—中药学]