二阶导数光谱法测定复方吴茱萸碱纳米乳中吴茱萸碱  

Determination of evodiamine in compound evodiamine nanoemulsion by second order derivative spectrophotometry

作　　者：柳珊[1] 谭群友[2] 吴建勇[1] 尹华峰[1] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室,重庆400016 [2]第三军医大学大坪医院野战外科研究所胸外科,重庆400042

出　　处：《中成药》2012年第4期670-673,共4页Chinese Traditional Patent Medicine

基　　金：教育部博士点基金资助项目(20095503120008);重庆市教育委员会资助项目(首批高等学校优秀人才资助;KJ090308)

摘　　要：目的建立二阶导数光谱法测定复方吴茱萸碱纳米乳中吴茱萸碱。方法通过有机溶剂法破乳,采用二阶导数光谱法,于288 nm和291 nm波长处测定峰谷差值(P288-V291),测定复方吴茱萸碱纳米乳中吴茱萸碱。结果吴茱萸碱在5.2～20.8μg/mL质量浓度范围内与二阶导数峰谷差值呈良好的线性关系;回归方程为:Y=0.081 2X+0.003 5(r=0.999 7);平均回收率为99.60%、RSD为1.04%(n=9);日内及日间精密度的RSD均小于1%(n=5)。结论本方法准确性高、精密度好、操作简单,可用于吴茱萸碱复方纳米乳中吴茱萸碱的测定。AIM To establish a method for determining evodiamine in compound evodiamine nanoemulsion. METHODS Ethanol was used for the emulsion-breaking and second order derivative spectrophotometry was applied to determining evodiamine content in nanoemulsion at wavelengths of 288 nm and 291 nm （P2ss - V291 ）＇ RESULTS The experiment showed a good linear relationship in the range of 5.2 - 20. 8 μg/mL for evodiamine. The regression equation was Y = 0.081 2 X ＋ 0. 003 5 （ r = 0. 999 7 ）. The average recovery was 99.60% and RSD was 1.04% （n = 9）. RSDs of the intra-day and inter-day precision were less than 1% （n = 5）. CONCLUSION The method is simple, quick and accurate. It is suitable for the determination of evodiaminein in compound evo- diamine nanoemulsion.

关 键 词：吴茱萸碱 纳米乳 定量测定 二阶导数光谱法 

分 类 号：R927.2[医药卫生—药学]