杜仲雄花HPLC指纹图谱及成分积累规律的研究  

HPLC fingerprint and accumulation rule of principal component in Eucommia ulmoides male flower

作　　者：叶东旭[1,2] 杜红岩[3] 李钦[1,2] 杜兰英[3] 付建敏[3] 

机构地区：[1]河南大学药学院,河南开封475004 [2]河南大学中药研究所,河南开封475004 [3]中国林业科学研究院经济林研究开发中心,河南郑州450003

出　　处：《中成药》2012年第4期706-709,共4页Chinese Traditional Patent Medicine

基　　金：国家林业公益性行业科研专项(201004029);国家"948"项目(2008-4-66);河南省科技攻关重点项目(102102310188)

摘　　要：目的建立杜仲雄花的HPLC指纹图谱,分析杜仲雄花中成分的积累规律。方法采用Ameritech C18(250 mm×4.6 mm,5μm)色谱柱,甲醇-0.05%磷酸溶液梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长238 nm。结果对不同日期采集的杜仲雄花的指纹图谱进行评价,生成对照指纹图谱,确定了22个共有峰。杜仲雄花的成分积累规律为京尼平苷酸含有量花蕾期最低,至盛花期最高。绿原酸含有量花蕾期最高,始花期最低,末花期上升。京尼平苷含有量始花期最低,盛花期最高。结论杜仲雄花的盛花期是兼顾质量和产量的最佳采摘期。AIM To establish HPLC fingerprint of Eucommia ulmoides male flower and analyze its accumulation of principal component. METHODS RP-HPLC method was used for the determination. The separation was performed on an Ameritech Cls column （250 mm× 4. 6 mm, 5 μm）, the mobile phase was methanol with 0. 05% phosphoric acid in the gradient elution mode. The flow rate was 1.0 mL/min. The temperature of column was maintained at 25 ℃. The detective wavelength was set at 238 rim. RESULTS The fingerprint of samples from different picking times were used to get the reference chromatogram composed of twenty-two common peaks. Geni- posidic acid content was the lowest at the bud stage, and the maximum content was in full bloom stage. The chlorogenic acid was the highest at the bud stage, and the lowest at the initial flowering stage, and increased at the end of flowering phase. The content of geniposide was the lowest at the initial flowering stage, and the highest in full bloom stage. CONCLUSION The result shows that picking time of Eucommia ulmoides male flower in full bloom would be recognized as the optimal one.

关 键 词：杜仲雄花 高效液相色谱法 指纹图谱 成分积累规律 

分 类 号：R284.1[医药卫生—中药学]