剑桥滤片-液-液捕集系统各部分烟气捕集物的细胞毒性比较  

Comparison on Cytotoxicity of Cigarette Smoke Components Trapped at Each Stage in Cambridge Filter-Solvent-Solvent Systems

作　　者：夭建华[1] 米其利[1] 耿永勤 杨叶昆[1] 黄海涛[1] 李雪梅[1] 缪明明[1] 唐萍[1] 

机构地区：[1]云南烟草科学研究院

出　　处：《烟草科技》2012年第4期51-55,共5页Tobacco Science & Technology

基　　金：国家烟草专卖局基金项目"烟用添加剂安全性评价关键技术研究"(110200902060);云南省应用基础研究项目"烟草添加剂安全性评价的体外毒理学方法研究"(2009ZC190M);云南省烟草化学创新团队建设项目"云南烟草科学研究院烟草化学省创新团队"(2009CI014);云南省中烟工业公司项目"烟草行业烟用添加剂安全性评价体系建设"(2009JC01)

摘　　要：为考察烟气捕集在卷烟主流烟气体外细胞毒性检测中的作用,采用3种捕集系统:剑桥滤片-pH7.0 PBS-丙酮、剑桥滤片-pH7.2 PBS-乙酸乙酯和剑桥滤片-pH7.4 PBS-70%甘油捕集同一卷烟主流烟气的粒相物和气相物,用中性红细胞毒性法检测了各部分烟气捕集物对中国仓鼠卵巢细胞(CHO)的毒性作用,气相色谱-质谱法检测了3种有机溶剂烟气捕集物的化学成分。结果表明:①细胞毒性的大小顺序为:剑桥滤片捕集物>PBS捕集物>有机溶剂捕集物;②在第2级捕集物中,pH7.4 PBS烟气捕集物的细胞毒性显著大于pH7.0和pH7.2 PBS烟气捕集物;③在第3级捕集物中,70%甘油烟气捕集物的细胞毒性最大,丙酮和乙酸乙酯的较小,且二者比较接近;④3种有机溶剂捕集的烟气气相物部分主要是醛类、酯类、烷烃和苯系物,70%甘油捕集了较多的烷烃和苯系物。剑桥滤片捕集的烟气冷凝物反映了部分烟气对CHO细胞的中性红细胞毒性,剑桥滤片-pH7.4 PBS溶液-70%甘油捕集的烟气物质可以较全面地反映烟气的细胞毒性。In order to investigate the effects of cigarette smoke trapping on in vitro cytotoxicity test,3 kinds of trapping systems,including Cambridge filter-pH7.0 PBS-acetone,Cambridge filter-pH7.2 PBS-ethyl acetate and Cambridge filter-pH7.4 PBS-70% glycerol,were used to collect particulate matter and gas phase from the mainstream smoke of the same cigarettes,the cytotoxicity of smoke trapped at each stage in the systems to CHO（Chinese Hamster ovary） cells was tested by neutral red method,and the chemical compositions in the matter trapped by organic solvents were analyzed by gas chromatography-mass spectrometry.The results indicated that： 1） the cytotoxicity of the matter trapped by Cambridge filter was more significant than that by PBS and organic solvent;2） for the 2nd stage trapped matter,the cytotoxicity of that trapped by pH7.4 PBS was significantly higher than that by pH7.0 and pH7.2;3） for the 3rd stage trapped matter,the cytotoxicity of that trapped by the 70% glycerol was significant,that by acetone and ethyl acetate was less and close.The vapor phase components trapped by the 3 organic solvents were mainly aldehydes,esters,alkanes and benzene analogues.There were more alkanes and benzene analogues captured by 70% glycerol.In conclusion,the smoke condensate trapped by Cambridge filter reflected part of neutral red cytotoxicity of smoke to CHO cell,while that trapped by Cambridge filter-pH7.4 PBS-70% glycerol more completely reflected the cytotoxicity of smoke.

关 键 词：烟气捕集 剑桥滤片 磷酸缓冲液 丙酮 乙酸乙酯 甘油 细胞毒性 中性红 

分 类 号：TS411.2[农业科学—烟草工业]