反复IVF失败患者卵泡液代谢组学的检测分析  

Metabolomic Profile Analysis of Human Follicular Fluid in Repeated IVF Failure Patients Using Gas Chromatography/Mass Spectrometry

作　　者：夏兰[1] 赵晓明[1] 孙赟[1] 洪燕[1] 郑菊芬[1] 向祖琼[1] 姚宁[1] 赵磊文[1] 郭毅[1] 胡双纲[1] 

机构地区：[1]上海交通大学医学院附属仁济医院生殖医学科,上海200001

出　　处：《生殖与避孕》2012年第4期248-253,共6页Reproduction and Contraception

基　　金：上海高校选拔培养优秀青年教师科研专项基金项目

摘　　要：目的:探讨与卵子发育潜能相关的代谢物质。方法:运用气相色谱-质谱仪(GC-MS)回顾性分析13例反复IVF失败(RIF组)和15例同期第1次行IVF-ET成功妊娠患者(对照组)的卵泡液代谢组学。结果:用偏最小二乘法分析样本主成分,结果显示RIF组和对照组的主成分积分值集中分布于2个不同区域,且反复IVF失败组较对照组有11种氨基酸含量显著升高,而二元羧酸、胆固醇及部分有机酸含量显著降低。结论:升高的缬氨酸、苏氨酸、异亮氨酸、半胱氨酸、丝氨酸、脯氨酸、丙氨酸、苯丙氨酸、赖氨酸、甲硫氨酸、鸟氨酸,和降低的二元羧酸、胆固醇可能成为预测卵子发育潜能的代谢标志物。Objective： To explore the metabolomic profile which can access the oocyte developmental capacity.Methods： The follicular fluid（FF） samples were collected from 13 patients with repeated IVF failure（RIF） and 15 patients who got pregnant at the first IVF-ET theropy.Gas chromatography/mass spectrometry（GC-MS） was utilized to obtain the metabolomic information.Results： The PCA-DA scores showed that the different samples were scattered into two different regions,20 metabolites were detected differently between the two groups.Conclusion： Elevated amino acid such as valine,threonine,isoleucine,cysteine,serine,proline,alanine,phenylalanine,lysine,methionine,ornithine and the decline of dual-carboxylic acid and cholesterol may predict the oocyte developmental capacity.

关 键 词：反复IVF失败 卵泡液 代谢组学 胚胎发育潜能 

分 类 号：R714.8[医药卫生—妇产科学]