低氧对体外受精-胚胎移植胚胎发育潜能的影响  

Effect of Low Oxygen Concentration on in-vitro Embryonic Development in in vitro Fertilization-Embryo Transfer

作　　者：刘迎春[1] 邢琼[1] 周平[1] 纪冬梅[1] 陈蓓丽[1] 苏天红[1] 章志国[1] 曹云霞[1] 

机构地区：[1]安徽医科大学第一附属医院生殖医学中心,合肥230022

出　　处：《生殖与避孕》2012年第4期269-272,共4页Reproduction and Contraception

摘　　要：目的:探讨低氧环境(体积分数5%O2)对体外受精-胚胎移植(in vitro fertilization-em-bryo transfer,IVF-ET)中胚胎发育潜能及临床结局的影响。方法:将接受IVF-ET长方案治疗的265名不孕症患者随机分为研究组(n=156):患者取卵后受精及整个胚胎培养过程全部在三气培养箱(体积分数5%O2)中进行,对照组(n=109):患者取卵后受精及整个胚胎培养过程全部在常规培养箱(体积分数20%O2)内进行,所有患者均移植授精第2日或第3日胚胎。比较组间受精率、正常受精率、卵裂率、正常卵裂率、优质胚胎率、可用胚胎率、生化妊娠率、临床妊娠率和异位妊娠率。结果:组间患者年龄、不孕年限、体质量指数、基础性激素、获卵数、成熟卵数、授精至移植时间和移植胚胎数均无统计学差异(P>0.05)。研究组受精率(84.4%)、正常受精率(72.0%)、卵裂率(97.6%)、优质胚胎率(43.3%)和可用胚胎率(72.5%)均显著高于对照组(分别为80.8%、68.7%、96.1%、35.1%、59.5%)(P<0.01或P<0.05),研究组与对照组正常卵裂率(97.7%vs 98.0%)、生化妊娠率(50.0%vs 39.4%)、临床妊娠率(44.9%vs 35.8%)、异位妊娠率(8.6%vs 12.8%)均无统计学差异(P>0.05)。结论:低氧环境(5%O2)似乎能够提高胚胎的发育潜能,获得更多优质胚胎和可用胚胎。Objective： To investigate the effect of low oxygen concentration on in vitro fertilization（IVF） embryonic development and clinical outcome.Methods： A total of 265 patients received infertile treatment by using a long induced ovulation protocol.The patients were allocated into experimental group（156 patients） and control group（109 patients）.In experimental group,the entire duration from insemination to day 2 or day 3 embryo transfer,which contained fertilization and cleavage embryo culture process were in three-gas incubators containing 5% O2,while oocytes and cleavage embryos were in common incubators with 20% O2in the control.Results： There was no significant difference in patients’age,duration of infertility,body mass index（BMI）,basal sexual hormone,the number of matured oocytes,the number of embryos for transfer and the day from in vitro fertilization to transfer embryo between two groups.The fertilization rate（84.4% vs 80.8%,P〈0.01）,normal fertilization rate（72.0 % vs 68.7%,P〈0.05）,cleavage rate（97.6% vs 96.1%,P〈0.01）,good-quality embryo rate（43.3% vs 35.1%,P 0.01） and available embryo rate（72.5% vs 59.5%,P〈0.01） in experimental group were significantly higher than those in the control,but the normal cleavage rate（97.7% vs 98.0%）,biochemistrial pregnancy rate（50.0% vs 39.4%）,clinical pregnancy rate（44.9% vs 35.8%） and ectopic pregnancy rate（8.6% vs 12.8%） were not statistically different.Conclusion： Low oxygen tension（5% O2） appears to improve embryonic development by increasing the number of good-quality embryos and available embryos.

关 键 词：氧浓度 体外受精 胚胎发育 

分 类 号：R714.8[医药卫生—妇产科学]