Genistein对受辐射L-02人肝细胞增殖和DNA损伤的影响  

Effects of genistein on proliferation and DNA damage of irradiated human liver L-02 cells

作　　者：类春燕[1] 沈秀华[2] 张乃宁[1] 丛峰松[3] 马俐君[4] 宋立华[1,5] 

机构地区：[1]上海交通大学农业与生物学院食品科学与工程系,上海200240 [2]上海交通大学医学院营养系,上海200025 [3]上海交通大学生命科学技术学院实验教学中心,上海200240 [4]上海交通大学医学院附属仁济医院长宁分院肿瘤科,上海200050 [5]上海交通大学陆伯勋食品安全研究中心,上海200240

出　　处：《第二军医大学学报》2012年第4期359-363,共5页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30972460);上海市自然科学基金(09ZR1415400)~~

摘　　要：目的探讨金雀异黄素(genistein,GEN)对L-02人肝细胞DNA辐射损伤的防护作用。方法 (1)L-02细胞经不同浓度(1、5、10、20μmol/L)GEN预处理24 h后,接受不同剂量(6、8、12、16、20 Gy)X线照射,利用MTT法检测细胞增殖情况。(2)L-02细胞经不同浓度GEN预处理后接受8 Gy(300 cGy/min)X线照射,利用单细胞凝胶电泳检测照射后不同时间(24、48、72 h)细胞DNA损伤情况。另设正常对照(N)、单纯GEN处理(G)及单纯照射(R)组作为参照。结果 (1)当照射剂量为6、8及12 Gy时,5μmol/L GEN预处理组细胞增殖率显著高于R组(P<0.05),而照射剂量为16 Gy和20 Gy时,各浓度GEN预处理组细胞增殖率与R组相比未见明显升高。(2)DNA损伤检测结果显示,N组和不接受照射的各浓度G组细胞均未见彗星拖尾。经8 Gy X线照射后24 h,R组及各浓度GEN预处理组彗星出现率均小于1%,各组间彗星尾长差异无统计学意义;照后48 h,R组彗星出现率达(24.2±1.2)%,彗星尾长达(283.6±22.3)μm,而各浓度GEN预处理组的彗星出现率和彗星尾长均较R组明显降低(P<0.05);照后72 h,R组彗星出现率较48 h明显下降(P<0.05)、彗星尾长明显缩短(P<0.05),1、5μmol/L GEN预处理组彗星出现率和彗星尾长仍明显低于R组(P<0.05),但10、20μmol/L GEN预处理组彗星出现率则明显升高(P<0.05),彗星尾长明显增长(P<0.05)。结论低浓度(1、5μmol/L)GEN能有效减轻较低剂量(8Gy)照射对L-02人肝细胞的DNA辐射损伤。Objective To investigate the radioprotective effects of genistein（GEN） against radiation-induced DNA damage in human liver cell line L-02.Methods（1）L-02 cells were treated with different concentrations of GEN（1,5,10,and 20 μmol/L） for 24 h,and then irradiated with X-ray at the doses of 6,8,12,16,and 20 Gy.Forty-eight hours after irradiation,MTT method was applied to examine the proliferation of L-02 cells.（2）L-02 cells were treated with different concentrations of GEN（1,5,10,and 20 μmol/L） for 24 h,and then irradiated with X-ray at the doses of 8 Gy（300 cGy/min）.Single cell gel electrophoresis was used to determine the DNA damage after radiation.Results（1）After irradiation with 6,8 and 12 Gy of X-ray,the cell proliferation rate of 5 μmol/L GEN-pretreated group was significantly increased compared to radiation alone（R） group（P〈0.05）.But no significant increase was observed in GEN-pretreated groups irradiated with 16 Gy and 20 Gy of X-ray compared with R group.（2）As for the DNA damage,no comet cells were detected in normal control group or all GEN-treated groups without irradiation.After irradiation with 8 Gy of X-ray for 24 h,comet incidences were less than 1% in all GNE-pretreated groups and R group,and comet tail length showed no significant difference between different groups.At 48 h after irradiation,the comet incidence of R group was（24.2±1.2）% and the comet tail length was（283.6±22.3） μm,while both comet incidence and tail length of GEN-pretreated groups were significantly lower than those of R group（P〈0.05）.The comet incidence and tail length of R group were significantly decreased 72 h after irradiation compared with 48 h after irradiation（P〈0.05）,and those in 1 μmol/L and 5 μmol/L GEN-pretreated groups were still significantly lower than those of R group（P〈0.05）,but the comet incidence and tail length of 10 μmol/L and 20 μmol/L GEN-pretreated groups were significantly increased compared to those of R group（P〈0.05）.Conclusi

关 键 词：金雀异黄素 肝细胞 X线 辐射损伤 DNA损伤 

分 类 号：R818.74[医药卫生—放射医学]