内皮素及其受体蛋白家族在糖尿病大鼠视网膜中的表达  

Expression of endothelins and endothelin receptors in retina of diabetic rats

作　　者：袁爱花[1,2] 蒋平[1] 梅妍[2] 羊惠君[2] 

机构地区：[1]上海交通大学医学院附属精神卫生中心遗传研究室,上海200030 [2]四川大学华西医学中心人体解剖与组织胚胎学教研室,成都610041

出　　处：《第二军医大学学报》2012年第4期436-440,共5页Academic Journal of Second Military Medical University

基　　金：上海市卫生局青年课题(2010Y030)~~

摘　　要：目的探讨内皮素-1(ET-1)、ET-2、ET-3、内皮素受体A(ETRA)、ETRB和内皮素转换酶(ECE)在8周糖尿病SD大鼠视网膜中的表达。方法采用限制性片段差异显示PCR(RFDD-PCR)技术建立正常和8周糖尿病大鼠视网膜基因差异表达谱,并以半定量RT-PCR和蛋白免疫印迹方法分别对两组间表达存在差异的6个基因(ET-1、ET-2、ET-3、ETRA、ETRB、ECE)的表达进行验证。结果 RFDD-PCR结果显示,糖尿病组ET-1、ET-2、ET-3、ETRA、ETRB和ECE表达上调。半定量RT-PCR结果和蛋白免疫印迹结果显示,6种基因和蛋白在糖尿病组的表达(相对光密度比值)高于正常组,表达差异具有统计学意义(P均<0.05,P均<0.01)。结论 ET-1、ET-2、ET-3、ETRA、ETRB和ECE在糖尿病视网膜中呈高表达,提示这6种基因可能参与了糖尿病视网膜的发病过程。Objective To investigate the expression of endothelin-1（ET-1）,ET-2,ET-3,endothelin receptor A（ETRA）,ETRB and endothelin coverting enzyme（ECE） in the retinas of 8-week-old diabetic SD rats.Methods The retinal gene expression profiles of healthy and 8-week-old diabetic rats were constructed with restriction fragment differential display polymerase chained reaction（RFDD-PCR）,and the differential expression of ET-1,ET-2,ET-3,ETRA,ETRB and ECE was verified using semi-quantitative RT-PCR and Western blotting analysis.Results The results of RFDD-PCR showed that the expression of ET-1,ET-2,ET-3,ETRA,ETRB and ECE was up-regulated in diabetic retina.The results of semi-quantitative RT-PCR and Western blotting analysis showed that the expression levels of the six genes and proteins（relative D ratio） in diabetic group were significantly higher than those in the normal retinas（P〈0.05 and P〈0.01,respectively）.Conclusion The expression of ET-1,ET-2,ET-3,ETRA,ETRB and ECE is up-regulated in diabetic retina,suggesting that the six genes may be involved in the pathgenesis of diabetic retina.

关 键 词：糖尿病视网膜病变 内皮素 内皮素受体 内皮素转换酶 

分 类 号：R587.26[医药卫生—内分泌]