化红胶囊对去势大鼠骨质疏松的影响  

Protective effect of Huahong capsule on ovariectomy-induced bone loss in rats

作　　者：周继刚 薛冰洁[2] 曹丹[2] 周创 陈茂华 汪鋆植[2] 

机构地区：[1]宜昌市中医医院,湖北宜昌44300 [2]三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌443000

出　　处：《中国医院药学杂志》2012年第8期587-590,共4页Chinese Journal of Hospital Pharmacy

基　　金：湖北省卫生厅中西医结合项目(编号:2010Z-Y23)

摘　　要：目的:探讨化红胶囊对去势大鼠骨质疏松的影响及作用机制。方法:取3月龄SD雌性大鼠经去势手术或者是假阳性手术后,喂以去大豆特殊饲料7周以诱导骨丢失。经去势手术大鼠进一步分为化红胶囊组(1.0,0.5,0.25 g.kg-1)、模型组、己烯雌酚组,连续给药12周。检测大鼠血清的骨钙素、雌二醇、尿中脱氧吡啶啉含量,以及椎骨末端骨小梁的变化情况。结果:化红胶囊能降低大鼠血清中骨钙素以及尿液中脱氧吡啶啉含量,提高血清中雌二醇含量,与模型组相比,1.0g.kg-1,0.5 g.kg-1剂量组差异有显著性(P<0.05或P<0.01)。骨小梁的形态学观察及数据分析显示,与模型组相比,化红胶囊可以提高骨小梁面积百分比,与模型组相比,1.0,0.5 g.kg-1剂量组差异有显著性(P<0.05或P<0.01)。结论:化红胶囊可以降低去势大鼠骨转换率和骨吸收程度,提高骨小梁平均面积百分比,是一种潜在的治疗绝经后骨质疏松药物,其作用机制之一可能与化红胶囊雌激素样作用有关。OBJECTIVE To study the protective effect of Huahong capsule on ovariectomy-induced bone loss in rats and its mechanisms of actions. METHODS Three-month-old female Sprague-Dawley rats were ovariectomized（OVX） and fed on special diet without soybeen for 7 weeks to induce bone loss. The OVX rats were further divided into five subgroups respectively, with Huahong capsule（1.0,0. 5,0. 25 g·kg^-1 ）, vehicle （OVX） , diethylstilbestrol for 12 weeks. The concentrations of osteocalcin, estradiol in serum, deoxypyridinoline in urine, the variation of proximal end of the vertebrae were determined. RESULTS Compared with model group, administration of Huahong capsule decreased serum osteocalcin and urine deoxypyridinoline and increased serum E2 content. And significant differences were observed in the Huahong capsule treated rats （1.0 g·kg^-1 and 0. 5 g·kg^-1）. The Huahong capsule groups increased the thickness and the area percentage of the vertebra of the trabecular bone of ovariectomized rats, the effect of 1.0 g·kg^-1 and 0. 5 g·kg^-1 were significantly different compared with OVX group. CONCLUSION Huahong capsule has a definite protective effect on bone loss in ovariectomized rats by inhibiting bone turnover and bone resorption and increasing the area percentage of the trabecular bone of the vertebra, Huahong capsule is a potential drug that prevents postmenopausal osteoporosis. The mechanism may be related with its estrogewlike effects.

关 键 词：化红胶囊 湖北海棠 骨质疏松 雌激素 

分 类 号：R285.5[医药卫生—中药学]