拟南芥甲基结合蛋白基因AtMBP11启动子在种子形成和萌发中的功能鉴定  

Functional Identification of the Promoter from Arabidopsis Methly-binding Domain Proteins AtMBP11 in Seed

作　　者：冀芦沙[1] 于守超[1] 赵燕[1] 肖庆振[1] 王曰文[1] 

机构地区：[1]聊城大学植物分子生物学重点实验室,聊城252059

出　　处：《生物技术通报》2012年第4期74-79,共6页Biotechnology Bulletin

基　　金：聊城大学博士启动基金项目(31805)

摘　　要：为研究拟南芥甲基结合蛋白基因AtMBP11在种子形成和萌发过程中的调控模式,克隆拟南芥AtMBP11启动子,将其替换植物表达载体pBI121的35S启动子序列,转入拟南芥基因组中。转基因拟南芥后代卡那霉素抗性发生分离,选取具有3 1分离比的后代自交,产生纯合的具有单拷贝插入的后代。转基因后代GUS染色结果表明,新克隆的MBP启动子控制基因在种子、花药和花粉中高效表达。通过对AtMBP11核心启动子缺失分析表明,G-box元件是主要功能元件。To investigate the regulation mechanisms involved in the development and germination of the Arabidopsis seeds,the plant expression vector pBI121 was constructed with the AtMBP11 promoter driving β-glucuronidase（GUS） gene which was transformed into the Arabidopsis genome with flower-dip infiltration.In T2 generation,in which the kanamycin resistance segregated,the individual plant with 3 1 segregation ratio was selected and then selfed fertilized to produce single copy homozygous transformant.Subsequently,GUS staining profile of the transformants showed that staining was mainly appeared in germinated seeds,pollen and anther.The deletion analysis showed that the G-box is cole function element of the AtMBP11 promoter.

关 键 词：拟南芥 启动子 甲基结合蛋白(MBP) 

分 类 号：Q943[生物学—植物学]