内蒙古白绒山羊IGF-IR基因cDNA克隆及组织表达特异性分析  

Cloning and Tissue-specific Expression Pattern of IGF-IR Gene cDNA in Inner Mongolia Cashmere Goat

作　　者：宝梅英[1] 赵荷雅[1] 杨娇馥[1] 鲍文蕾[1] 李彬[1] 杨军 侯鑫[1] 王志钢[1] 

机构地区：[1]内蒙古大学生命科学学院,呼和浩特010021 [2]内蒙古呼和浩特市环境科学研究所,呼和浩特010030

出　　处：《生物技术通报》2012年第4期103-107,共5页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目(30860191;31160469)

摘　　要：旨在克隆内蒙古白绒山羊IGF-IR基因并分析其基本表达模式。采用RT-PCR克隆基因,将得到的IGF-IR基因cDNA片段的核苷酸序列及其编码的氨基酸序列进行生物信息学分析。半定量RT-PCR进行组织特异性表达检测。获得了内蒙古白绒山羊IGF-IR基因3'端编码区2 118 bp的cDNA序列(JN200823),编码705个氨基酸残基。核苷酸序列与牛的IGF-IR(XM606794.3)基因同源性为98%,相应的氨基酸序列同源性为99%。SMART分析表明,推导出的编码蛋白具有跨膜域,酪氨酸激酶催化域。半定量RT-PCR检测表明,IGF-IR基因在绒山羊脑、胰腺、肝、肾组织中均有表达。The present study aims at cloning the code sequence fragment of IGF-IR gene cDNA from Inner Mongolia cashmere goat and analyzing its tissue-specific expression pattern.IGF-IR gene cDNA fragments were cloned by RT-PCR.The nucleotide sequence was analyzed by BLAST while amino acid sequence was analyzed by online soft wares SMART.The tissue-specific expression pattern of IGF-IR gene was analyzed by semi-quantitative RT-PCR.The cloned 3＇ terminal 2 118 bp IGF-IR gene cDNA（JN200823）from Inner Mongolia Cashmere goat was a part of coding region,encoding 705 amino acid residues.The nucleotide sequence shares 98% identity with Bos tuarus IGF-IR gene（XM606794.3）and the amino acid sequence shares 99% identity.Analysis with SMART suggested that the deduced encoding protein contained a transmembrance domain and a TyrKc domain.The IGF-IR gene was shown to express in brain,pancreas,liver and kidney.

关 键 词：内蒙古白绒山羊 IGF-IR 组织特异性表达 

分 类 号：S827[农业科学—畜牧学]