通窍活血汤改善拟血管性痴呆大鼠学习记忆作用的研究  

Activity of Tongqiao Huoxue Decoction on the Learning and Memory Ability of Rats with Vascular Dementia in Rats

作　　者：葛朝亮[1,2] 汪宁[2] 

机构地区：[1]安徽医科大学第一附属医院药剂科,合肥230022 [2]安徽中医学院,安徽省中药研究与开发重点实验室,合肥230038

出　　处：《中国药学杂志》2012年第8期590-594,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30973979);安徽省优秀青年科技基金(10040606Y17);康缘中医药科技创新基金项目(KYCX2010007)

摘　　要：目的研究通窍活血汤对拟血管性痴呆(vascular dementia,VD)大鼠学习记忆作用及脑皮质中乙酰胆碱含量的影响,探讨通窍活血汤对拟血管性痴呆大鼠的作用机制。方法采用颈总动脉(CCA)注射混合血栓诱导剂法制作拟血管性痴呆大鼠模型。通过八臂迷宫检测大鼠工作记忆错误次数、参考记忆错误;光镜(HE染色)观察拟血管性痴呆大鼠脑组织海马CA1区病理形态的改变;酶联免疫吸附试验(ELISA)方法检测拟血管性痴呆大鼠脑皮质中乙酰胆碱的含量。结果通窍活血汤高、中剂量能显著减少拟血管性痴呆大鼠的工作记忆错误次数、参考记忆错误次数(P<0.01);明显改善拟血管性痴呆大鼠脑组织海马CA1区病理形态学异常;显著提高拟血管性痴呆大鼠脑皮质中乙酰胆碱的含量(P<0.01)。结论通窍活血汤能明显改善拟血管性痴呆大鼠的学习、记忆能力。其作用机制可能与改善拟血管性痴呆大鼠脑组织海马CA1区的椎体细胞的病理形态的异常,提高拟血管性痴呆大鼠脑皮质中乙酰胆碱的含量有关。OBJECTIVE To study the activity of TQHXD on the learning and memory ability of rats with vascular dementia（VD） and its effects on the content of Ach in cerebral cortex.And to investigate the action mechanism of TQHXD on VD in rats.METHODS VD model was made by common carotid artery injection of a co-thrombus inducer.The 8-arm radial maze experiment was adopted to evaluate the times of working memory errors and reference memory errors.The changes of the pathological area in hippocampus CA1 were observed by optical microscope.Enzyme-linked immunosorbent assay was used to determine the concentration of Ach in rat＇s cerebral cortex.RESULTS High and middle dose of TQXHD significantly reduce the times of working memory errors and reference memory errors（P0.01）,definitely improved the anormalies of pathological area in hippocampal CA1,and significantly increased the content of Ach in cerebral cortex（P0.01）.CONCLUSION TQHXD can significantly ameliorate the learning and memory ability of in VD rats.The mechanism may be related to the improvement of the vertebral body cells anomalies in the hippocampal CA1 region and increasing the content of the Ach in cerebral cortex.

关 键 词：通窍活血汤 血管性痴呆 海马CA1区 乙酰胆碱 

分 类 号：R965[医药卫生—药理学]