大鼠肠道菌对虎杖苷的生物转化研究  

Biotransformation of Polydatin by Rat Intestinal Bacteria

作　　者：张文婷[1] 黄琴伟[1] 向智敏[1] 程维明[1] 

机构地区：[1]浙江省食品药品检验所,杭州310004

出　　处：《中国药学杂志》2012年第8期631-634,共4页Chinese Pharmaceutical Journal

摘　　要：目的利用大鼠肠内厌氧细菌对虎杖中主要单体虎杖苷进行生物修饰研究,观察虎杖苷在大鼠体内及体外经肠内菌代谢后的变化并确定代谢物。方法生物样品经处理后分别进行LC-MS/MS及HPLC检测分析。结果从虎杖苷的大鼠肠内厌氧细菌培育物中检出2个化合物,即虎杖苷及大鼠肠内厌氧细菌代谢产物白藜芦醇;大鼠ig虎杖苷1 500 mg.kg-1后提取4 h胃肠道内容物,证明肠道存在虎杖苷和代谢产物白藜芦醇。结论虎杖苷可被大鼠肠内菌代谢,其代谢模式为虎杖苷→白藜芦醇。OBJECTIVE To study the gastrointestinal metabolic transformation of polydatin,one of the main bioactive components in Rhizoma Polygoni,thus to figure out the possible biological modification exerted by rat intestinal bacteria in vitro and in vivo,and to determine the possible metabolites.METHODS The biological samples were analyzed by high performance liquid chromatography（HPLC） and liquid chromatography mass spectrometer（LC-MS/MS） after treatments,respectively.RESULTS Two main compounds,polydatin and resveratrol,were identified in rat gastrointestinal contents 4 h after the ig administration of polydatin 1 500 mg·kg-1.CONCLUSION This study revealed that polydatin could be metabolized to resveratrol by rat intestinal bacteria through desugarization.

关 键 词：虎杖苷 肠内菌代谢 白藜芦醇 生物转化 

分 类 号：R969[医药卫生—药理学]