异丙酚对LPS诱导BV-2小胶质细胞IL-1β和TNF-α释放的影响及TLR4在其中的作用  

Effect of propofol on IL-1β and TNF-α release from BV-2 microglia cells induced by lipopolysaccharide and the role of Toll-like receptor 4

作　　者：宿明艳[1] 桂波[1] 钱燕宁[1] 

机构地区：[1]南京医科大学第一附属医院麻醉科,210029

出　　处：《中华麻醉学杂志》2012年第2期198-200,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81070276）

摘　　要：目的评价异丙酚对LPS诱导BV-2小胶质细胞IL-1β和TNF-α释放的影响及Toll样受体4（TLR4）在其中的作用。方法将体外培养的BV-2小胶质细胞接种于96孔培养板中，采用随机数字表法，将其随机分为4（n=12）：对照组、LPS组、异丙酚组和LPS＋异丙酚组。LPS组加入LPS 1μg／ml孵育24h；异丙酚组加入异丙酚30μmol／L孵育24h；LPS＋异丙酚组同时加入LPS1μg／ml和异丙酚30μmol／L孵育24h。于孵育6h时，采用ELISA法检测细胞上清液TNF-α浓度，以此反映TNF-α的释放量，采用RT-PCR法测定TLR4mRNA表达；于孵育24h时，采用ELISA法检测细胞上清液IL-1β浓度，以此反映IL-1β的释放量，采用Western Blot法检测TLR4蛋白表达。结果与C组比较，LPS组和LPS＋异丙酚组IL-1β和TNF-α的释放量升高，TLR4mRNA及其蛋白表达上调（P〈0．05）；与LPS组比较，LPS＋异丙酚组IL-1β和TNF—α的释放量降低，TLR4mRNA及其蛋白表达下调（P〈0．05）。结论异丙酚可抑制LPS诱导BV-2小胶质细胞IL-1β和TNF-α的释放，其机制与抑制TLR4的表达有关。Objective To investigate the effect of propofol on IL-1β and TNF-α release from BV-2 micro- glia ceils induced by lipopolysaceharide （LPS） and the role of Toll-like receptor 4 （TLR4）. Methods BV-2 microglia cells were seeded in 96-well plates and randomly divided into 4 groups （ n = 12 each） ： control group, LPS group, propofol group （group P） and LPS ＋ propofol group. In group LPS, the cells were incubated with LPS 1 μg/ml for 24 h. In group P, the cells were incubated with propofol 30μmol/L for 24 h. In group LPS ＋ propofol, the cells were incubated with LPS 1 μg/ml and propofol 30 μmol/L for 24 h. The concentrations of TNF-α （at 6 h of incubation） and IL-1β （at 24 h of incubation） in the superoatant were detected by ELISA. TLR4 mRNA expression was detectedat at 6 h of incubation by RT-PCR. TLR4 protein expression was detected at 24 h of incubation by Western blot. Results Compared with control group, IL-1β and TNF-α release was significantly increased, and the expression of TLR4 mRNA and protein up-regulated in groups LPS and LPS ＋ propofol （ P 〈 0.05）. Compared with group LPS, IL-1β and TNF-α release was significantly decreased, and the expression of TLR4 mRNA and protein down-regulated in group LPS ＋ propofol （P 〈 0.05 ）. Conclusion Propofol can inhibit IL-1β and TNF-α release from BV-2 microglia cells induced by LPS and inhibition of TLR4 expression may be involved in the mechanism.

关 键 词：异丙酚 脂多糖类 白细胞介素1Β 肿瘤坏死因子α 小胶质细胞 TOLL样受体4 

分 类 号：R965[医药卫生—药理学]