乳化异氟醚预处理对大鼠局灶性脑缺血再灌注时血小板活化因子及其受体表达的影响  

Effects of emulsified isoflurane preconditioning on expression of platelet-activating factor and its receptor in brain during focal cerebral ischemia-reperfusion in rats

作　　者：丁兆霞[1] 史炯[1] 王志萍[2] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,221002 [2]南京医科大学附属无锡市第一人民医院麻醉科

出　　处：《中华麻醉学杂志》2012年第2期221-224,共4页Chinese Journal of Anesthesiology

基　　金：江苏省卫生厅资助项目（H200723）;江苏省医学重点人才资助项目（RC2007093）;江苏省高校自然科学重大基础研究项目（08KJA320001）

摘　　要：目的探讨乳化异氟醚预处理对大鼠局灶性脑缺血再灌注时血小板活化因子（PAF）及其受体表达的影响。方法健康成年雄性SD大鼠32只，体重250—300g，采用随机数字表法，将大鼠随机分为4组（n=8）：假手术组（S组）、缺血再灌注组（I／R组）、乳化异氟醚组（EI组）和脂肪乳组（LE组）。采用线栓阻塞大脑中动脉2h行再灌注的方法制备大鼠局灶性脑缺血再灌注损伤模型。于缺血前24h，S组和I／R组腹腔注射生理盐水10．5ml／kg，EI组和LE组分别给予8％乳化异氟醚和30％脂肪乳10．5ml／kg。再灌注12h时进行神经功能缺陷评分，随后每组处死4只大鼠，采用TIC染色法测定脑梗死体积，每组取其余4只大鼠，采集静脉血样，采用ELISA法测定血浆PAF浓度，随后处死大鼠，分离海马和皮层，采用Western blot法测定PAF受体的表达。结果与S组比较，其余3组神经功能缺陷评分评分、脑梗死体积百分比、血浆PAF浓度、海马和皮层PAF受体表达升高（P〈0．01）；与I／R组比较，EI组上述指标降低（P〈0．05），LE组上述指标差异无统计学意义（P〉0．05）。结论乳化异氟醚预处理可减轻大鼠局灶性脑缺血再灌注损伤，其机制与抑制PAF及其受体表达有关。Objective To investigate the effects of emulsified isoflurane preconditioning on the expression of platelet-activating factor （PAF） and PAF receptor during focal cerebral ischemia-reperfusion （I/R） in rats. Methods Thirty-two healthy adult male SD rats weighing 250-300 g were randomly divided into 4 groups （ n = 8 each）： group sham operation （group S）; group I/R; group emulsified isoflurane preconditioning（ group El） and group lipid emulsion （group LE）. Focal cerebral I/R was induced by 2 h occlusion of middle cerebral artery followed by reperfusion in groups I/R, EI and LE. 8% emulsified isoflurane 10.5 ml/kg and 30% lipid emulsion 10.5 ml/kg were injected intraperitoneally at 24 h before cerebral ischemia in groups EI and LE respectively. The neurologic deficit score （NDS） （0 = no deficit, 4 = unable to control） was evaluated at 12 h of reperfusion. Ve- nous blood samples were collected for measurement of plasma PAF concentration. The animals were then sacrificed and their brains removed for determination of infarct size （by TYC staining） and PAF receptor expression in hippocampus and cerebral cortex （by Western blot）. Results Focal cerebral I/R significantly increased NDS, the infarct size, plasma PAF concentration and PAF receptor expression in cerebral cortex and hippocampus in group I/R as compared with group S. Emulsified isoflurane preconditioning significantly attenuated the focal cerebral I/R induced above changes in group El as compared with group I/R, but there was no significant difference between groups I/R and LE. Conclusion Emulsified isofiurane preconditioning can attenuate focal cerebral I/R injury by inhibiting PAF and PAF receptor expression.

关 键 词：异氟醚 脂肪乳剂 静脉注射用 再灌注损伤 血小板活化因子 受体 细胞 表面 脑 

分 类 号：R965[医药卫生—药理学]