异丙酚不同时机给药对大鼠海马缺氧复氧损伤神经元胞浆细胞色素c的影响  

Effects of different time administration of propofol on cytochrome c in cytoplasm in rat hippocampal neurons with hypoxia-reoxygenation injury

作　　者：于灵芝[1] 张娜[1] 姜丽[3] 黄鲁斌[2] 张刚[1] 石秀芳[1] 

机构地区：[1]山东大学附属济南市中心医院疼痛科,250013 [2]山东大学附属济南市中心医院麻醉科,250013 [3]河北省人民医院麻醉科

出　　处：《中华麻醉学杂志》2012年第2期225-227,共3页Chinese Journal of Anesthesiology

基　　金：第三批济南留学归国人员创业基金（03104）

摘　　要：目的评价异丙酚不同时机给药对大鼠海马缺氧复氧损伤神经元胞浆细胞色素c浓度（CtyC）的影响。方法原代培养大鼠海马神经元，采用随机数字表法，将其随机分为5组（n=5）：对照组（c组）、缺氧复氧损伤模型组（M组）和异丙酚不同时机给药组（Ⅰ组、Ⅱ组和Ⅲ组）。采用缺氧6h再复氧的方法制备海马神经元缺氧复氧损伤模型。I组、Ⅱ组和Ⅲ组分别于缺氧前、复氧即刻和复氧2h（T0-2）时加入异丙酚至终浓度20μmol／L。各组分别于T1,2和复氧24h（T3）时观察细胞凋亡情况，检测胞浆CytC的浓度。结果与c组相比，M组T1-3时、Ⅰ组、Ⅱ组和Ⅲ组T1,2时细胞胞浆Ctyc浓度升高（P〈0．05）；与M组相比，Ⅰ组，T1-3时、Ⅱ组和Ⅲ组T1,2时细胞胞浆Cryc浓度降低（P〈0．05）；与Ⅰ组相比，Ⅱ组和Ⅲ组，T1,2时细胞胞浆Ctyc浓度升高（P〈0．05）；与Ⅱ组相比，Ⅲ组T2时细胞胞浆Ctye浓度升高（P〈0．05）。不同时机异丙酚给药组细胞凋亡数目较M组明显减少。结论异丙酚不同时机给药可减少线粒体CtyC释放到胞浆，抑制海马神经元凋亡，减轻缺氧复氧损伤，缺氧前给药效果较好。Objective To investigate the effects of different time administration of propofol on cytochrome c （Cyt c） in the cytoplasm in rat hippocampal neurons with hypoxia-reoxygenation （H/R） injury. Methods Pri- mary cultured hippocampal neurons were randomly divided into 5 groups （ n = 5 each） ： control group （group C）, model of H/R injury group （group M）, and different time administration of propofol groups （group Ⅰ , Ⅱ , Ⅲ） In groups M, Ⅰ , Ⅱ and Ⅲ, the neurons were exposed to 95% N2 ＋ 5% CO2 for6 h followed by 12 h reoxygenation. In groups Ⅰ ,Ⅱ , Ⅲ, propofol was added to the culture medium before hypoxia, immediately after reoxygenation and at 2 h of reoxygenation （T0-2 ） respectively, with the final concentration of 20 μmol/L. The cell apoptosis was observed at T1.2 and at 24 h of reoxygenation （T3 ） and the concentration of Cyt c in the cytoplasm was detected at T1-3 . Results Compared with group C, the concentration of Cyt c in the cytoplasm was significantly increased at T3 in group M and at T1,2 in groups Ⅰ , Ⅱ , Ⅲ （P 〈 0.05）. Compared with group M, the concentration of Cyt c in the cytoplasm was significantly decreased at T3 in group I and at T1,2 in groups IT and Ⅲ （ P 〈 0.05）. The concentration of Cyt c in the cytoplasm was significantly higher at T2 in groups Ⅱ and Ⅲ than in group Ⅰ, and at T2 in group m than in group Ⅱ （P 〈 0.05）. The neuronal apoptosis was significantly decreased in groups Ⅰ , Ⅱ and Ⅲ as compared with group M. Conclusion Different time administration of propofol can reduce the mitochondrial Cyt c release to the cytoplasm, inhibit apoptosis in hippocampal neurons, and reduce H/R injury in rats, with better effect when given before hypoxia.

关 键 词：二异丙酚 海马 细胞低氧 细胞色素C类 

分 类 号：R965[医药卫生—药理学]