HSP70基因多态性与焦炉工外周血淋巴细胞凋亡的易感性研究  

Relationship between HSP70 Genes Polymorphisms and Susceptibility of Lymphocyte Apoptosis in Peripheral Blood of the Cock-oven Workers

作　　者：胡志鹏[1,2] 李卫星[3] 张红明[4] 张慧芳[1] 常珊珊[1] 郑金平[1] 王保平[4] 李治玉[4] 

机构地区：[1]山西医科大学公共卫生学院毒理教研室,山西太原030001 [2]长治市妇幼保健院 [3]太原市疾病预防控制中心射线防治科 [4]山西焦化集团有限公司职业病防治所

出　　处：《环境与健康杂志》2012年第4期306-310,共5页Journal of Environment and Health

基　　金：国家自然科学基金(30872137);山西省自然科学基金(2009011049-1);山西医科大学科技创新基金(01200701)

摘　　要：目的探讨焦炉工人HSP70基因多态性与外周血淋巴细胞凋亡率的关系。方法于2008年7—8月,在某焦化厂选取341名工人作为研究对象,按照工人班后尿1-羟基芘(1-OHP)的浓度,分为低暴露组(<1.52μmol/mol cr)、中暴露组(1.52～3.88μmol/mol cr)、高暴露组(>3.88μmol/mol cr)。采用流式细胞仪测定淋巴细胞凋亡率,以限制性片段长度多态性(PCR-RFLP)方法分析焦炉作业工人外周血淋巴细胞HSP70-1基因G190C、HSP70-2基因A1267G和HSP70-hom基因T2437C基因多态性。结果携带HSP70-hom基因T/C+C/C基因型中、高暴露组工人淋巴细胞早期凋亡率均高于低暴露组(P<0.05)。携带HSP70-1 G/C+C/C、HSP70-2 A/G+G/G和HSP70-hom T/C+C/C基因型高暴露组工人淋巴细胞早期凋亡阳性率均高于低暴露组(P<0.05)。HSP70-2基因A/G+G/G基因型与高暴露环境之间存在次相乘模型的正向交互作用(γ>1,OReg=11.273,95%CI=2.244～56.641);HSP70-hom基因T/C+C/C基因型与高暴露环境之间存在超相乘模型的正向交互作用(γ>1,OReg=3.718,95%CI=1.293～10.693)。低、中、高暴露组GAC、CGT单倍型者淋巴细胞早期凋亡阳性率逐渐增加(P趋势<0.05)。结论 HSP70-2基因A/G+G/G基因型、HSP70-hom基因T/C+C/C基因型与高暴露环境的交互作用可增加淋巴细胞凋亡的危险度。GAC和CGT单倍型是淋巴细胞早期凋亡阳性率增加的危险因素。Objective To study the feasibility of HSP70 genes polymorphisms as biological marker of lymphocytes in peripheral blood of the coke-oven workers injury susceptibility by investigating the relationship between HSPTO genes polymorphisms and lymphocyte apoptosis. Methods A total of 341 coke-oven workers were investigated in a eoking plant by the questionnaires from July to August,2008. 1-hydroxypyrene （1-OHP）concentration in worker＇s urine after work was determined by high performance liquid chromatography. Subjects were divided into low （〈1.52 μmol]mol cr）,medium （1.52- 3.88μmol/mol cr） and high exposure group（〉3.88 μmol/mol er） according to the 1-OHP concentration. Lymphocyte apoptosis in peripheral blood was determined using the flow cytometry. HSP70-1 G19OC,HSP70-2 A1267G,HSP70-hom T2437C genotypes were analyzed by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique. Results The lymphoeytes apoptosis rates in moderate and high exposure groups were significantly different compared with low exposure group in HSP70-hom T/C＋C/C genotypes （P〈0.05）. Gene potymorphisms analysis showed that there were significantly difference of lymphoeytes apoptosis positive rates between high exposure group and low exposure group among HSP70-1 G/C＋ C/C, HSP70-2 A/G＋G/G and HSPTO-hom T/C＋C/C genotypes （P〈0.05）. Interactions between genes and environment factors showed different effects on lymphocytes apoptosis rates increased. S!gnificant positive-interaction was observed between HSP70-2 A/G＋G/G genotype and high exposure environmental （＂/〉1,0R,s=l 1.273,95%CI=2.244-56.641）, which appeared to act in a sub-multiplicative model. There was also observed significant positive-interaction between HSP70-horn T/C ＋C/C genotype and high exposure environmental （γ〉1,OR,,=3.718,95%CI=1.293-10.693）, which appeared to act in a super- multiplieative model. The positive rate of lymphocyte apoptosis in the low,moderate and high exposure group wor

关 键 词：焦炉工人 热休克蛋白70 基因多态性 细胞凋亡 

分 类 号：R181.3[医药卫生—流行病学]