血红蛋白在TiO_2-石墨烯/离子液体/壳聚糖纳米复合膜修饰电极上的直接电化学  

Direct Electrochemistry of Hemoglobin at TiO_2-Graphene/Ionic Liquid/Chitosan Nanocomposite Modified Electrode

作　　者：韩金土[1] 黄克靖[1] 王兰[1] 杨豪[1] 

机构地区：[1]信阳师范学院化学化工学院,信阳464000

出　　处：《化学通报》2012年第4期330-334,共5页Chemistry

基　　金：河南省高校科技创新人才基金项目(2010HASTIT025);河南省科技创新杰出青年基金项目(104100510020)资助

摘　　要：基于TiO2-石墨烯、离子液体和壳聚糖复合膜修饰玻碳电极制备了一种新型的电化学传感器。用循环伏安法研究了血红蛋白在该修饰电极上的直接电化学行为。结果表明,该纳米复合膜能有效地促进血红蛋白在电极上的直接电子转移,保持其生物催化活性。该传感器对H2O2具有良好的催化性能。H2O2的电流响应信号与其浓度在20～860μmol/L范围内呈良好的线性关系,检出限为0.1μmol/L(S/N=3)。传感器具有良好的稳定性和重现性。A novel electrochemical biosensor was fabricated based on TiO2-graphene/ionic liquid/chitosan composite film.The direct electrochemical behavior of hemoglobin at the modified electrode was investigated by cyclic voltametry（CV）.The results showed that the biocomposite film can effectively improve the direct electron transfer rate and well maintain electrocatalytic activity.The sensor has good catalytic performance for H2O2.The concentrations of H2O2 show good linear relationship with the current respond signal in the range of 20~860 μmol/L.The limit of detection is 0.1μmol/L（S/N=3）.The sensor exhibits good stability and reproducibility.

关 键 词：TiO2-石墨烯 离子液体 血红蛋白 直接电化学 

分 类 号：O646[理学—物理化学]