刚果红显色法测定羧甲基壳聚糖含量  

Determination of Carbomethyl-chitosan by Color Reaction with Congo-Red

作　　者：刘树恒[1] 王立霞[1] 刘长霞[1] 王瑜[1] 

机构地区：[1]沧州师范学院化学与环境科学系,沧州061000

出　　处：《化学通报》2012年第4期376-378,共3页Chemistry

摘　　要：在pH=9.0的NH3-NH4Cl缓冲溶液中羧甲基壳聚糖与刚果红形成稳定的红色物质;在530nm处具有最大吸收,其吸光度与羧甲基壳聚糖浓度在1.00～40.00mg/L范围内具有良好的线性关系,表观百分吸光系数ε=49.20mL/g.cm,方法用于羧甲基壳聚糖样品的测定,回收率在98.2%～101.8%之间,测定值的标准偏差在0.41%～2.5%之间。The experimental results showed that Congo Red and carbomethyl-chitosan produced a sensitive color reaction under the condition of pH=9 NH3-NH4Cl buffer solution.The biggest absorption wavelength was at 530nm.It was found that the absorbance（A） had good linear relationship with concentration of carbomethyl-chitosan from 1.00 mg/L to 40.00 mg/L.The method can be applied to determine carbomethyl-chitosan content in compound samples.

关 键 词：羧甲基壳聚糖 刚果红 显色反应 测定 

分 类 号：O636.1[理学—高分子化学] O657.3[理学—化学]