HPLC同时测定穿心莲药材及其注射液中3种内酯类成分的含量  

Simultaneous Determination of 3 Components of Diterpenoids in Andrographis Paniculata and Chuanxinlian Injection by HPLC

作　　者：董海娟[1] 张尊建[1] 

机构地区：[1]中国药科大学分析测试中心药物质量与安全预警教育部重点实验室,南京210009

出　　处：《中国现代应用药学》2012年第4期349-352,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：中央高校基本科研业务费专项资金项目(JKQ2009010)

摘　　要：目的利用HPLC同时测定穿心莲药材及其注射液中穿心莲内酯、脱水穿心莲内酯和新穿心莲内酯的含量。方法色谱柱为Shimadzu Shim-pack VP-ODS柱（4.6 mm×150 mm,5μm）,以甲醇-水（53∶47）洗脱,流速：1 mL.min？1,柱温：25℃,检测波长225 nm处检测穿心莲内酯,检测波长205 nm处检测脱水穿心莲内酯与新穿心莲内酯。结果穿心莲药材及其注射液中3种内酯类成分能达到很好分离,线性关系良好（r≥0.999 5）;平均加样回收率为99.6%~101.2%,RSD〈3%。结论本方法灵敏、准确、可靠、重复性好,可用于穿心莲药材及其注射液的质量评价与3种内酯类成分的快速测定。OBJECTIVE To develop a quantitative method for simultaneous determination of andrographolide,deoxyandrographolide and neoandrographolide in Andrographis paniculata and Chuanxinlian injection by HPLC.METHODS A Shimadzu Shim-pack VP-ODS C18 column（4.6 mm×150 mm,5 μm） was used with CH3OH and water（53∶47） as mobile phase.The flow rate was 1 mL·min 1,the column temperature was 25 ℃,the detection wavelength of andrographolide was set at 225 nm and the detection wavelength of deoxyandrographolide and neoandrographolide was set at 205 nm.RESULTS The 3 active components of diterpenoids were in baseline separation.The linearity of this method was good（r≥0.999 5）,and the average recoveries were 99.6% 101.2%,RSD3%.CONCLUSION The method is sensitive,accurate,reliable and repeatable,which can be used for quality evaluation of Andrographis paniculata and its injection,and for determination of 3 active components of diterpenoids quickly.

关 键 词：穿心莲 内酯类成分 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R917.101[医药卫生—中医学]