肺炎支原体感染儿童血外周血辅助性T细胞17型和白细胞介素-17变化及其临床意义  

Changes of T Help Cell 17 and Interleukin-17 in Childre n With Mycoplasma Pneumoniae Infection and Its Clinical Significance

作　　者：宋磊[1] 赵小冬[1] 杨小红[1] 孔炜[1] 周峰[2] 汪晓莺[3] 

机构地区：[1]江苏省南通市第一人民医院儿科,江苏南通226001 [2]江苏省南通市第一人民医院检验科 [3]南通大学免疫教研室

出　　处：《中华妇幼临床医学杂志（电子版）》2012年第2期140-143,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：江苏省南通市市级社会事业科技创新与示范计划指令性课题(HS2011040)~~

摘　　要：目的探讨外周血辅助性T细胞(Th)17型和白细胞介素(IL)-17在肺炎支原体(MP)急性感染儿童体内变化及其临床意义。方法选择2009年6月至2010年12月在南通市第一人民医院儿科接受住院治疗并确诊为肺炎支原体肺炎(MPP)的32例患儿为研究对象,并纳入MPP组,平均年龄为(9.3±2.5)岁。将其进一步按照感染症状分为MPP亚组1(普通肺炎症状,n=18)和MPP亚组2(肺炎合并肺外症状,n=14);选择同期于该院门诊行健康体检儿童纳入对照组(n=26)(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试对象监护人的知情同意,并与之签署临床研究知情同意书)。MPP组与对照组、MPP亚组1与MPP亚组2受试者的年龄、性别等比较,差异无统计学意义(P>0.05)。常规分离外周血单核细胞(PBMC),采用流式细胞术(FACS)检测各组外周血Th17;酶联免疫吸附法(ELISA)检测血清细胞因子IL-17水平。结果①MPP亚组1与MPP亚组2的外周血Th17频率均高于对照组[(2.05±0.65)%vs.(1.21±0.36)%,(2.98±0.72)%vs.(1.21±0.36)%;P<0.01];MPP亚组2外周血Th17频率均高于MPP亚组1[(2.98±0.72)%vs.(2.05±0.65)%,P<0.01]。②MPP亚组1与MPP亚组2血清IL-17水平均高于对照组[(11.62±5.37)pg/mLvs.(6.45±1.62)pg/mL,(17.34±7.45)pg/mLvs.(6.45±1.62)pg/mL;P<0.01];MPP亚组2血清IL-17水平高于MPP亚组1[(11.62±5.37)pg/mLvs.(17.34±7.45)pg/mL;P<0.01]。结论 MP感染患儿体内Th17频率明显改变,且伴炎症性细胞因子IL-17水平升高,Th17频率异常在MP感染的发病机制中起重要作用。Objective To study the change and clinical signif icances of helper T cell（Th） 17 and interleukin（IL）-17 in children with Myc oplasma pneumoniae（MP） infection.Methods A total of 32 children with MP pneumonia（MPP） were recruited in the Department of Pediatrics of Na ntong People＇s Hospital from June 2009 to December 2010.They were selected int o MPP group with the average age of（9.3±2.5） years old.They were fu r ther divided into MPP sub-group 1（symptom of pneumonia,n=18） and MPP sub-group 2（symptom with lesion of exo-lung,n= 14）.At the same time,a total of 26 he alth children who underwent physical examination were selected into control grou p.The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Panzhihua Second People＇s Hospital.Informed consent was obta ined from each participate.The peripheral blood mononuclear cells were isolated,then frequency of Th17 were determined by flow cytometry（FACS）.Serum conten ts of IL-17 were detected by enzyme linked immunosorbent assay（ELISA）.Results ① The frequencies of Th17 cells of MP infection children in MPP sub-group 1 and MPP sub-group 2 were obviously higher than those in control group [（2.05±0.65）% vs.（1.21±0.36）%,（2.98±0.72）% vs.（1.21±0.36）%,P0.01],the frequency of Th17 cells of MPP sub-group 2 was higher than that in MPP sub-group 1[（2.98±0.72）% vs.（2.05±0.65）%,P 0.01].② The serum levels of IL-17 in MP infection group were higher than tho se in control group [（11.62±5.37） pg/mL vs.（6.45±1.62） pg/mL,（17.34±7.45） pg/mL vs.（6.45±1.62） pg/mL,P0.01],MPP sub-group 2 was higher than that in MPP sub-group 1[（11.62±5.37） pg/mL vs.（17.34±7.45） pg/m L,P0.01].Conclusions Abnormal frequencies of Th17 and IL-17 levels play a key role in the pathogenic mechanism of MP infection in chi ldren.

关 键 词：肺炎支原体 辅助性T细胞17型 白细胞介素-17 

分 类 号：R725.6[医药卫生—儿科]