酚妥拉明和地塞米松对吸入性损伤大鼠肾脏损伤的保护作用及其机制  

The effect of phentolamine and dexamethasone on renal injury in rats with severe smoke inhalation injury

作　　者：孙志刚[1] 鲁开化[2] 齐顺贞[1] 曹景敏[2] 李占军[1] 

机构地区：[1]白求恩国际和平医院烧伤整形科,河北石家庄050082 [2]第四军医大学西京医院整形科外科中心,陕西西安710032

出　　处：《中国危重病急救医学》2000年第4期232-235,共4页Chinese Critical Care Medicine

摘　　要：目的 :观察酚妥拉明和地塞米松对重度吸入性损伤大鼠肾脏损害的保护作用及其机制。方法 :72只大鼠随机分为 3组。对照组腹腔注射生理盐水 ,A组腹腔注射酚妥拉明 ,B组腹腔注射地塞米松。另取 6只非致伤大鼠作伤前值测定 ,再取 6只致伤大鼠 ,伤后 30分钟作伤后值测定。结果 :对照组尿素氮 (BUN)、丙二醛(MDA)、MDA/超氧化物歧化酶 (SOD)及组织含水量均增高 ,血中 NO水平升高 ,肾组织中 NO水平变化不如血中明显 ,SOD、ATP水平明显降低。 A组和 B组中 BUN和组织及血中的 NO明显低于对照组。 A组和 B组中 ATP、SOD均明显高于对照组 ,组织含水量低于对照组。结论 :酚妥拉明与地塞米松均可能通过调节机体的一氧化氮水平进而提高机体抗氧化能力和能量代谢 ,从而对重度吸入性损伤后大鼠的肾脏起保护作用。Objective:To observe the protective effect of phentolamine and dexamethasone on renal injury in rats with severe smoke inhalation injury,and to explore their potential mechanism.Methods:72 rats were divided into three groups:control group (saline treatment),phentolamine treatment group (group A),and dexamethasone treatment group (group B).In addition,6 animals without inhalation injury served as normal controls,and another 6 animals subjected to inhalation injury were sacrificed at 30 minutes.Results:In the control group,levels of blood urea nitrogen (BUN),malondialdehyde (MDA),MDA/superoxide dismutase (SOD),and tissue water content increased,and plasma NO levels increased while renal SOD,ATP contents obviously decreased.Blood BUN and plasma and tissue nitric oxide (NO) levels in both group A and group B were markedly lower than those in control group.Contents of SOD and ATP in both groups were higher compared with the controls,together with decrease in tissue water content in two treatment groups.Conclusions:Both phentolamine and dexamethasone can regulate renal NO level through improvement of antioxidation ability and energy metabolism,which may be associated with the protective effect on the renal function in rats with severe smoke inhalation injury.

关 键 词：吸入性损伤 酚妥拉明 地塞米松 肾脏 治疗 

分 类 号：R96[医药卫生—药理学]