银屑病模型与脾虚证银屑病病证结合模型相关指标的差异  

The differences of related laboratory parameters between a mouse model of psoriasis with spleen-deficiency syndrome and psoriasis

作　　者：郭姗姗[1] 郭菲[1] 刘红霞[2] 

机构地区：[1]新疆医科大学研究生学院,乌鲁木齐830011 [2]新疆医科大学附属中医医院皮肤科,乌鲁木齐830011

出　　处：《新疆医科大学学报》2012年第4期416-419,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81060296/H2709)

摘　　要：目的探讨银屑病模型与脾虚证银屑病病证结合小鼠模型相关实验室指标的差异。方法昆明种小鼠108只,100%大黄煎液灌胃小鼠7d以建立脾虚模型(模型2组),第5～7d脾虚造模的同时腹腔注射已烯雌酚进行银屑病造模(模型1组),另设空白组,每组36只,处死小鼠后,观察两组脾虚相关指标血清褪黑激素(MT)与银屑病相关指标血浆内皮素-1、可溶性E选择素及细胞增殖性核抗原(PCNA),分析其差异。结果 (1)脾虚相关指标:空白组、模型1组MT分别与模型2组比较差异有统计学意义(P<0.05),模型1组与空白组无明显差异(P>0.05);(2)银屑病相关指标:空白组与模型1组、2组血浆内皮素-1、可溶性E选择素水平以及阴道上皮PCNA表达差异均有统计学意义(P<0.05);模型1组与模型2组血浆内皮素-1、可溶性E选择素水平差异有统计学意义(P<0.05),阴道上皮PCNA表达无明显差异(P>0.05)。结论银屑病模型与银屑病病证结合模型在脾虚相关指标及银屑病相关指标方面均有不同程度的差异。Objective Explore the differences of related laboratory parameters between a mouse model of psoriasis with spleen-deficiency syndrome and psoriasis syndrome only.Methods Mice were 108,and by 100% rhubarb decoction gavage in mice 7 d to build the the spleen-deficiency model（model 2）,which the 5~7 d spleen-deficiency were intraperitoneally injected with diethylstilbestrol to build psoriasis model（model 1）,a separate blank group（n=36）,mice were sacrificed after the observation of the relevant indicators of the two groups Spleen concentrations of melatonin（MT） and psoriasis-related indicators of plasma endothelin-1,soluble E-selectin and proliferating cell nuclear antigen（PCNA） analysis of the differences.Results（1）Spleen-deficiency index： control group model 1 MT compare with the model 2 respectively,the indicators was statistically significant（P0.05）,model group and blank group was no significant difference（P0.05）.（2）Psoriasis-related indicators： control group and model groups,plasma endothelin-1,soluble E-selectin levels and vaginal epithelial PCNA expression differences were statistically significant（P0.05）.Model 1 and the model 2 plasma endothelin-1,soluble E-selectin levels was statistically significant（P0.05）,vaginal epithelial expression of PCNA was no significant difference（P0.05）.Conclusion Psoriasis model and which with Spleen-deficiency Syndrome model in Spleen indicators and psoriasis indicators have different degrees of difference.

关 键 词：银屑病 脾虚证 病证结合模型 实验研究 

分 类 号：R751[医药卫生—皮肤病学与性病学]