贝母素乙逆转SGC7901/VCR细胞株多药耐药的初步研究  

Reversal effect of peiminine on gastric cancer multidrug resistance

作　　者：张裴[1] 顾政一 孙玉华 聂勇战 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011 [2]新疆维吾尔自治区药物研究所,乌鲁木齐830004 [3]西京消化病医院肿瘤生物学国家重点实验室,西安710032

出　　处：《新疆医科大学学报》2012年第4期452-456,共5页Journal of Xinjiang Medical University

摘　　要：目的研究贝母素乙对胃癌耐药细胞SGC7901/VCR的逆转及诱导凋亡作用。方法采用四甲基唑蓝法(MTT)检测贝母素乙的细胞毒性及耐药逆转效果;采用流式细胞仪检测细胞内阿霉素(ADR)蓄积以及细胞凋亡率的变化。结果贝母素乙可剂量依赖性的的抑制亲本细胞系SGC7901和耐药细胞系SGC7901/VCR的细胞增殖,20μg/mL贝母素乙能够显著提高SGC7901/VCR细胞对化疗药(长春新碱、阿霉素、顺铂和5-氟尿嘧啶)的敏感性及细胞内ADR的浓度,贝母素乙和5-氟尿嘧啶(5-Fu)联合用药后能诱导细胞凋亡,细胞凋亡率随着药物浓度的增加而逐渐增加,处理48h后的凋亡率最高达44.5%。结论贝母素乙能够作为胃癌多药耐药逆转剂逆转MDR,其机制可能与减少药物外排和诱导细胞凋亡相关。Objective To investigate the effect of Peiminine on gastric cancer multidrug resistance（MDR）.Methods The growth inhibition on SGC7901 and SGC7901/VCR of peiminine was examined by MTT assay.The intracellular adriamycin（ADR） accumulation was detected by flow cytometry.Cell apoptosis was analyzed by fluorescence staining.Results Peiminine could inhibit SGC7901 and SGC7901/VCR cells growth.Furthermore,20 μg/mL Peiminine could enhance the sensitivity of chemotherapeutic drugs and increase intracellular ADR accumulation.FCM assay suggested that Peiminine could induce SGC7901/VCR cells apoptosis in dose depend.The apoptosis rate of up to 44.5% at 48 h.Conclusion Peiminine would to be a promising anti-MDR agent in gastric cancer by reducing drug efflux and affecting cell apoptosis and so on.

关 键 词：贝母素乙 多药耐药 逆转 凋亡 

分 类 号：R966[医药卫生—药理学]