蝎毒多肽促进创伤皮肤溃疡愈合的实验研究  

Study on healing wound skin ulcers of scorpion venom peptides

作　　者：张曼[1] 顾少菊[1] 孔天翰[1] 黄劭[1] 覃媛[1] 

机构地区：[1]广州医学院蛇毒研究所,广东广州510182

出　　处：《中国医药导报》2012年第10期22-23,共2页China Medical Herald

基　　金：广东省广州市属高校科技计划项目(项目编号:1014);广东省医学科研基金(项目编号:A2009247);广州医学院科学研究项目(项目编号:03-k-39;03-k-21;2006ZR013)

摘　　要：目的研究蝎毒多肽促进皮肤溃疡愈合的作用及其机制。方法 NIH小鼠60只,采用创伤溃疡模型,分成五组,分别为外用无菌生理盐水组(空白组)、外用重组牛碱性成纤维细胞生长因子(bFGF,贝复济)组(150 AU/cm2)、蝎毒多肽高、中、低浓度治疗组(4×10-3、4×10-4、4×10-5mg/mL),给药的3、5、7、9 d测量创面面积,计算溃疡愈合率;采用液体试管法和琼脂稀释法观察体外抑菌作用。结果随给药时间延长,蝎毒多肽治疗组溃疡愈合率逐渐升高,与空白组比较,差异有统计学意义;蝎毒多肽对金黄色葡萄球菌和铜绿假单胞菌的最小抑菌浓度(MIC)为0.125 0 mg/mL。结论蝎毒多肽具有促进皮肤溃疡愈合及体外抑菌的作用。Objective To study the effects of scorpion venom peptides on curing skin ulcers.Methods The wound skin ulcers model was used in 60 NIH mice.Mice were administered saline,bFGF150 AU/cm^2,scorpion venom peptides 4×10^-3,4×10^-4,4×10^-5 mg/mL,respectively.The ulcer healing rates were tested on post-healing day 3,5,7,9.The minimum inhibitory concentration（MIC） with staphylococcus aureus and pseudomonas aeruginosa were tested by using broth dilution method and agar plate methods.Results The ulcer healing rates were increased compared to control group with statistically significant differences.The MIC of scorpion venom peptides was 0.125 0 mg/mL.Conclusion Scorpion venom peptides can heal the wound skin ulcers and have inhibitory effects of bacteria in vitro.

关 键 词：蝎毒多肽 皮肤溃疡 体外抑菌 

分 类 号：R331.22[医药卫生—人体生理学]