大孔吸附树脂纯化黄连总生物碱工艺研究  

Studies on purification process of total alkaloids from Coptis chinensis by macroporous adsorption resin

作　　者：吕子明[1,2] 刘文娟 王永 刘晓燕 梁俊清 屠鹏飞[1] 

机构地区：[1]北京大学药学院天然药物及仿生药物国家重点实验室,北京100191 [2]北京以岭药业有限公司,北京100027

出　　处：《中国医药导报》2012年第12期41-43,共3页China Medical Herald

基　　金：国家科技重大专项课题(项目编号:2011ZX09401-020)

摘　　要：目的研究大孔吸附树脂分离和纯化黄连总生物碱的工艺条件。方法以总生物碱和小檗碱的吸附量和解吸率为考察指标,对9种不同类型的树脂进行评价。并对优选出的大孔树脂纯化黄连总黄生物碱时的工艺条件及参数进行研究。结果 D141大孔吸附树脂的动态吸附分离效果最好,上样药液浓度为30 mg药材/mL,径高比为1∶8,吸附流速为2 BV/h,除杂溶剂为水,除杂体积为2 BV,除杂流速为1 BV/h,洗脱溶剂为50%乙醇,洗脱体积为4 BV,洗脱流速为3 BV/h。通过大孔吸附树脂分离纯化后,终产品中总生物碱的纯度为67.71%。结论该工艺合理、可行,适合工业生产。Objective To investigate the process of isolation and purification of total alkaloids from Coptis chinensis by macroporous adsorption resin.Methods The absorption amount and desorption ratio were taken as the investigation index to evaluated 9 kinds of resins.And the process and parameters of purification of total alkaloids from Coptis chinensis by optimized macroporous adsorption resin were studied.Results The dynamic adsorption and isolation effect of D141 type macroporous adsorption resin was the best,with the drug solution concentration as 30 mg of medicinal material/mL,ratio of diameter and height as 1∶8,velocity of absorption as 2 BV/h,solvent of removing impurities as water,volume of removing impurities as 2 BV,velocity of removing impurities as 1 BV/h,solvent of elution as 50% of ethanol,volume of elution as 4 BV,velocity of elution as 3 BV/h.The purity of total alkaloids in the final product was 67.71% after isolation and purification by macroporous adsorption resin.Conclusion The process is rational,feasible and suitable for industrial production.

关 键 词：黄连 总生物碱 小檗碱 大孔吸附树脂 纯化 

分 类 号：R282.71[医药卫生—中药学]