浙江省在鼠类中检测到伯氏疏螺旋体ospA基因  

The ospA genes of Borrelia burgdorferi sensu lato detected in rodents in Zhejiang province

作　　者：姜理平[1] 陆群英[1] 李钟梁[2] 张磊[1] 王复甦[1] 张政[1] 

机构地区：[1]浙江省疾病预防控制中心病原微生物所,浙江杭州310051 [2]磐安县疾病预防控制中心

出　　处：《中国媒介生物学及控制杂志》2012年第2期132-134,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的了解浙江省磐安县鼠中感染伯氏疏螺旋体的情况,研究以外膜蛋白A(OspA)为靶基因的PCR方法在浙江省的应用。方法利用PCR方法,检测磐安县128份鼠肝、脾标本中ospA基因特异片段;对所检测到的阳性结果进行序列测定,并进行分析。结果从128份鼠肝、脾标本中检测发现4例ospA基因阳性片段,其中3份来自黄毛鼠,1份来自大林姬鼠;核酸序列基本一致,与伯氏疏螺旋体法雷斯疏螺旋体高度相似。结论利用ospA基因能从标本中检测到伯氏疏螺旋体;浙江省部分山区存在鼠感染伯氏疏螺旋体的情况,其中伯氏疏螺旋体法雷斯疏螺旋体占优势。Objective To identify Borrelia burgdorferi sensu lato infection of rodents in Pan＇an county,Zhejiang province.Methods The ospA gene specific fragments from 128 mouse liver and spleen samples were detected using the PCR method and the positive samples were sequenced,followed by phylogenetic analysis.Results It was found that there were four ospA gene positive fragments out of the 128 samples,of which 3 fragments were obtained from Rattus losea and the other one was from Apodemus peninsulae.The 4 positive fragments shared high similarity with each other in terms of the sequences and shared high identities with B.valaisiana.Conclusion B.burgdorferi sensu lato can be detected from samples using the ospA gene.There exists B.burgdorferi sensu lato infection in rodents in some mountain areas of Zhejiang province,and B.valaisiana is found to be predominant.

关 键 词：鼠 莱姆病 伯氏疏螺旋体 外膜蛋白A基因 巢式聚合酶链反应 

分 类 号：R377[医药卫生—病原生物学]