二氧化碳嗜纤维菌对β-内酰胺类抗生素的耐药性分析及基因型检测  

The analysis of drug resistance and genotype detection of Capnocytophaga to β-lactam antibiotics

作　　者：陈东科[1] 胡付品 

机构地区：[1]卫生部北京医院检验科,北京市100730 [2]上海华山医院抗生素研究所,上海市200040

出　　处：《实用检验医师杂志》2011年第4期198-202,共5页Chinese Journal of Clinical Pathologist

基　　金：基金项目：国家自然科学基金（30872719）

摘　　要：目的 通过检测二氧化碳嗜纤维菌对β-内酰胺类抗生素的耐药性,了解其耐药机制.方法 收集3株二氧化碳嗜纤维菌,用纸片扩散法和Etest法检测二氧化碳嗜纤维菌对抗菌药物的敏感性,以Nitrocefin纸片法、三维法和PCR方法检测其β-内酰胺酶.结果 3株二氧化碳嗜纤维菌中有2株生痰二氧化碳嗜纤维菌,1株牙龈二氧化碳嗜纤维菌.3株菌对青霉素、氨苄西林、氨曲南、头孢噻肟、头孢他啶、头孢唑啉、头孢呋辛、头孢吡肟、头孢哌酮耐药,但对哌拉西林、亚胺培南、美罗培南、头孢西丁、氨苄西林/舒巴坦、头孢哌酮/舒巴坦、阿莫西林/克拉维酸、头孢他啶/克拉维酸、头孢噻肟/克拉维酸敏感.三维法试验结果显示3株二氧化碳嗜纤维菌所产的β-内酰胺酶能水解青霉素、氨苄西林、头孢噻肟、头孢他啶、头孢唑啉、头孢呋辛和头孢吡肟,但不能水解头孢西丁、亚胺培南和阿莫西林/克拉维酸.经PCR方法检测,3株二氧化碳嗜纤维菌的β-内酰胺酶和AmpC酶耐药基因均为阴性.结论 二氧化碳嗜纤维菌对常用抗菌药物尤其是β-内酰胺类抗生素均存在较高的耐药性,且其β-内酰胺酶可能存在新的基因型.Objective To understand the drug resistance mechanisms of Capnocytophaga by detecting its drug resistance to β-lactam antibiotics. Methods 3 strains Capnocytophaga was collected and susceptibil- ity testing of Capnocytophaga was carried out by disk diffusion method and Etest method. The β-lactamase was detected by Nitrocefin disk test, three-dimensional method and PCR method. Results There were 2 strains C. sputigena and 1 strain C.gingivalis. 3 strains of Capnocytophaga showed β-lactamase positive by Nitrocefin disk test. 3 strains of Capnocytophaga showed resistance to penicillin, ampicillin, aztreonam, cefotaxime, cef- tazidime, cefazolin, cefuroxime, cefepime and cefoperazone, but with sensitivity to pipercillin, imipenem, meraopenem, cefoxitin, ampicillin/sulbactam, cefoperazone/sulbactam, amoxicillin/clavulanic acid, ceftazidime/ clavulanic acid and cefotaxime/clavulanic acid. ^-lactamase produced by 3 strains of Capnocytophaga could be hydrolysised by penicillin, ampicillin, cefotaxime, ceftazidime, cefazolin, cefuroxime and cefepime, but with no effect on cefoxitin, imipenem and amoxicillin/clavulanic acid. The β-lactamase and AmpC enzyme genes of 3 strains Capnocytophaga were all negative by PCR method. Conclusion The drug resistance of Capnocytopha- ga to commonly used antibiotics especialy β-lactam antibiotics is higher, and there maybe a new genotype in β-lactamase

关 键 词：二氧化碳嗜纤维菌 Β-内酰胺酶 耐药性 

分 类 号：R978.11[医药卫生—药品]