补肾活血中药对糖尿病大鼠视路Nissl小体和Bcl-2的影响  

The Effect of Chinese Medicine of bushenhuoxue Therapy on Nissl Bodies and B Cell Lymphoma/Leukemia-2 in the Visual Pathway of experimental diabetic rats

作　　者：秦伟[1] 谢学军[2] 何宇[3] 李芳梅[4] 陈一兵[1] 翟楠[1] 

机构地区：[1]广州中医药大学附属中山医院,中山528400 [2]成都中医药大学眼科,成都610075 [3]四川大学华西医院西藏成办分院眼科,成都610042 [4]成都中医药大学附属绵阳中医院,绵阳621000

出　　处：《江西中医学院学报》2011年第6期65-67,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：国家自然科学基金(39600190)

摘　　要：目的:观察实验性糖尿病大鼠视路神经元组织形态学的病理改变,并探讨补肾活血中药复方防治实验性糖尿病大鼠视路损害的作用机理。方法:用免疫组化及Nissl染色结合图像分析半定量测定测定视网膜、外侧膝状体及视皮质的B淋巴细胞瘤/白血病-2基因(B cell lymphoma/leukemia-2,Bcl-2)的表达强度和Nissl小体的含量。结果:与正常对照组相比,阴性对照组大鼠视路三级神经元的Bcl-2的表达强度和Nissl小体的含量均明显降低(P<0.05或0.01);与阴性对照组相比,中药治疗组大鼠视网膜、外侧膝状体及是皮质的Bcl-2表达强度和Nissl小体的含量均明显增高,其中中药高剂量组最为明显(P<0.05或0.01)。结论:实验性糖尿病大鼠在高血糖状态持续6个月后,其视路神经元发生不同程度的退行性改变;补肾活血中药可一定程度抑制神经细胞凋亡,促进其存活,从而保护糖尿病大鼠视路神经元,减轻糖尿病性视路损害。Objective： To observe the histomorphological pathology change of the optic pathway neuron caused by the experimental diabetes,and to probe into the mechanism of bushenhuoxue Zhongyao Fufang to prevent from the lesion of the optic pathway of the experimental diabetic rats.Methods：The model of experimental diabetic rats was induced by streptozotocin（STZ）.Expression of B cell lymphoma/leukemia-2 and the content of Nissl bodies in retina,external geniculate body（EGB）and visual cortex（VC）were shown by immunohistochemical staining and toluidine blue-eosin staining,and were quantitated by image analysis system.Results： Compared with the normal control group,the positive staining of B cell lymphoma/leukemia-2 and the content of Nissl bodies in retina,EGB and VC were significangtly low in negative control group（P0.05 or 0.01）;Compared with the negative control group,the positive staining of Syn I and the content of Nissl bodies in retina,EGB and VC were significangtly high in all of the TCM groups,especially in the high dose group（P0.05 or 0.01）.Conclusion：The experimental diabetic rats which were in high blood glucose condition for 6 months,the optic pathway neuron had the varying degree of the recessive change;TCM of bushenhuoxue could increase the expression of B cell lymphoma/leukemia-2,accordingly it may be one of the important mechanisms of TCM for bushenhuoxue to relieve the damages of neurons in visual pathway with diabetic rats.

关 键 词：补肾活血 糖尿病 视路 Nissl小体 B淋巴细胞瘤/白血病-2基因 

分 类 号：R285.5[医药卫生—中药学]